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FrozONE: Quick Cell Nucleus Enrichment for Comprehensive Proteomic Analysis of Frozen Tissues

ABSTRACT: In this study, we introduce FrozONE, a workflow for the generation of deep nuclear proteomes by quick, robust and scalable nucleus enrichment from snap-frozen tissue material. We benchmarked our workflow’s capability to enrich and quantify nuclear proteins from multiple tissues and show its competitiveness with conventional ultracentrifugation-based nucleus enrichment strategies, while requiring shorter processing time, cheaper instrumentation and bypassing the need of fresh tissue processing, thereby simplifying experimental logistics and increasing scalability.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Liver, Kidney

SUBMITTER: Fabian Kliem

LAB HEAD: Maria Robles

PROVIDER: PXD050658 | Pride | 2024-12-05

REPOSITORIES: pride

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			Action	DRS
	20221029_FK_Exploris_Col_4_11_NucPrepTest_DIA_03.raw	Raw
	20221029_FK_Exploris_Col_4_11_NucPrepTest_DIA_07.raw	Raw
	20221029_FK_Exploris_Col_4_11_NucPrepTest_DIA_09.raw	Raw
	20230306_FK_EXP_MenetNucBrain1.raw	Raw
	20230306_FK_EXP_MenetNucBrain2.raw	Raw

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