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Effects of Copper on Legionella pneumophila Revealed via Viability Assays and Proteomics

ABSTRACT: This project examined changes in Legionella pneumophila strain 130b proteomics between copper exposed and control conditions.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Legionella Pneumophila 130b

TISSUE(S): Cell Suspension Culture

SUBMITTER: Keith Ray

LAB HEAD: Amy Jill Pruden-Bagchi

PROVIDER: PXD051926 | Pride | 2024-08-08

REPOSITORIES: Pride

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			Action	DRS
	210212_Didier_ControlEDTA_t0_01.raw	Raw
	210212_Didier_ControlEDTA_t0_02.raw	Raw
	210212_Didier_ControlEDTA_t0_03.raw	Raw
	210212_Didier_Control_t0_01.msf	Msf
	210212_Didier_Control_t0_01.msfView	Msf

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Publications

Effects of Copper on Legionella pneumophila Revealed via Viability Assays and Proteomics.

Song Yang Y Mena-Aguilar Didier D Brown Connor L CL Rhoads William J WJ Helm Richard F RF Pruden Amy A Edwards Marc A MA

Pathogens (Basel, Switzerland) 20240703 7

Cu is an antimicrobial that is commonly applied to premise (i.e., building) plumbing systems for Legionella control, but the precise mechanisms of inactivation are not well defined. Here, we applied a suite of viability assays and mass spectrometry-based proteomics to assess the mechanistic effects of Cu on L. pneumophila. Although a five- to six-log reduction in culturability was observed with 5 mg/L Cu2+ exposure, cell membrane integrity only indicated a <50% reduction ...[more]

PMID: 39057790

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