Proteomics

Dataset Information

0

Unique structural features of the human NuA4/TIP60 acetyltransferase and chromatin remodeling complex

ABSTRACT: The human NuA4/TIP60 co-activator complex, a fusion of the yeast SWR1 and NuA4 complexes, both incorporates the histone variant H2A.Z into nucleosomes and acetylates histones H4/H2A/H2A.Z to play crucial roles regulating gene expression and maintaining genome stability. Our cryo-EM studies show that within the NuA4/TIP60 complex, the EP400 subunit serves as an architectural scaffold holding the different functional modules in specific positions and giving rise to a novel arrangement of the ARP module. EP400 interacts with the TRRAP subunit using a footprint that overlaps with that of the SAGA acetyltransferase complex, thereby preventing the formation of a hybrid complex. Loss of the TRRAP subunit leads to mislocalization of NuA4/TIP60, resulting in the redistribution of H2A.Z and its acetylation across the genome,emphasizing the dual functionality of NuA4/TIP60 as a single macromolecular assembly.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Jie Luo  

LAB HEAD: Jie Luo

PROVIDER: PXD053207 | Pride | 2024-08-08

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
JL112922_120622_Epc1_100B.mzXML Mzxml
JL112922_120622_Epc1_1ug.mzXML Mzxml
JL112922_120622_Epc1_30B.mzXML Mzxml
JL112922_120622_Epc1_50B.mzXML Mzxml
JL112922_120622_Epc1_70B.mzXML Mzxml
Items per page:
1 - 5 of 15
altmetric image

Publications

The human nucleosome acetyltransferase of histone H4 (NuA4)/Tat-interactive protein, 60 kilodalton (TIP60) coactivator complex, a fusion of the yeast switch/sucrose nonfermentable related 1 (SWR1) and NuA4 complexes, both incorporates the histone variant H2A.Z into nucleosomes and acetylates histones H4, H2A, and H2A.Z to regulate gene expression and maintain genome stability. Our cryo-electron microscopy studies show that, within the NuA4/TIP60 complex, the E1A binding protein P400 (EP400) subu  ...[more]

Similar Datasets

Proteomics Unique structural features of the human NuA4/TIP60 acetyltransferase and chromatin remodeling complex
2024-08-08 | PXD053209 | Pride
Transcriptomics Unique structural features of the human NuA4/TIP60 acetyltransferase and chromatin remodeling complex [RNA-seq]
2024-08-06 | GSE261651 | GEO
Genomics Unique structural features of the human NuA4/TIP60 acetyltransferase and chromatin remodeling complex [ChIP-seq]
2024-08-06 | GSE261650 | GEO
Proteomics A structural model of the human mSWI/SNF (BAF) complex bound to the nucleosome informs disease mechanisms
2021-02-10 | PXD020992 | Pride
Proteomics TAF8 regions important for TFIID lobe B assembly are required for embryonic stem cell survival
2021-10-02 | PXD026575 | Pride
Transcriptomics Effects of MBTD1 on gene expression
2016-04-30 | GSE78024 | GEO
Transcriptomics Nucleosome free DNA- and acetyl histone-binding components recruit SWR1 to yeast promoters for H2A.Z replacement
2013-09-16 | E-GEOD-49183 | biostudies-arrayexpress
Proteomics Crosslinking Mapping of yeast Komagataella phaffii TFIID complex
2018-10-08 | PXD011092 | Pride
Proteomics Drosophila BAF complex crosslinking (
2018-10-18 | PXD010123 | Pride
Proteomics NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis
2021-12-20 | PXD030302 | Pride