Dataset Information

Mucosal Immunity of Puffer Fish against bacterial Infection

ABSTRACT: This study established immunological and particularly antibacterial proteins in the skin mucus of Obscure puffer against A. hydrophila infection. These proteins could be potential biomarkers to be studied for prevention of bacterial disease in fish. Overall, the study provides primary insights into the mucosal immune factors in the skin mucus of fish and recommends each protein for functional-based molecular studies.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Takifugu Obscurus

TISSUE(S): Skin, Mucus

DISEASE(S): Bacterial Sepsis

SUBMITTER: Xingkun Jin

LAB HEAD: Zhe Zhao

PROVIDER: PXD054382 | Pride | 2024-08-16

REPOSITORIES: Pride

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Publications

Bacterial infection-biased abundance of proteins in the skin mucus of obscure puffer (Takifugu Obscurus).

Dawar Farman Ullah FU Shi Yan Y Zhou Yu Y Jin Xingkun X Zhao Zhe Z

Comparative biochemistry and physiology. Part D, Genomics & proteomics 20240805

The skin mucus of fish is equipped with immunological and antimicrobial peptides that confer protection against invading pathogens. The skin mucus has been studied in fish however information regarding its immunological roles in bacterial infection is rare. This study highlighted the proteins and peptides in the skin mucus of Obscure puffer Takifugu obscurus that quantitatively altered against Aeromonas hydrophila infection. We infected the fish through bath immersion, intraperitonially, and tre ...[more]

PMID: 39116716

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Project description:Antimicrobial peptides (AMPs) constitute a broad range of bioactive compounds in diverse organisms, including fish. They are effector molecules for the innate immune response, against pathogens, tissue damage and infections. Still, AMPs from African Catfish, Clarias gariepinus skin mucus are largely unexplored despite their possible therapeutic role in combating antimicrobial resistance. In this study, African Catfish Antimicrobial peptides (ACAPs) were identified from the skin mucus of African Catfish, C. gariepinus. Native peptides were extracted from fish mucus scrapings in 10% acetic acid (v/v) and ultra-filtered using 5kDa molecular cut-off membrane. The extract was purified using C18 Solid Phase Extraction. The antibacterial activity was determined using the Agar Well Diffusion method and broth-dilution method utilizing Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922). Thereafter, Sephadex G-25 gel filtration was further utilized in bio-guided isolation of the most active fractions prior to peptide identification using Orbitrap Fusion Lumos Tribrid Mass Spectrometry. The skin mucus extracted from African Catfish from all the three major lakes of Uganda exhibited antimicrobial activity on E. coli and S. aureus. Lake Albert’s C. gariepinus demonstrated the best activity with the lowest MIC of 2.84 µg/mL and 0.71 µg/mL on S. aureus and E. coli respectively. Sephadex G-25 peak I mass spectrometry analysis alongside in silico analysis revealed seven short peptides (11-15 amino acid residues) of high antimicrobial scores (0.561-0.905 units). In addition, these peptides had a low molecular weight (1005.57-1622.05 Da), and had percentage hydrophobicity above 54%. Up to four of these antimicrobial peptides demonstrated α-helix structure conformation, rendering them amphipathic. The ﬁndings of this study indicate that novel antimicrobial peptides can be sourced from the skin mucus of C. gariepinus. Such antimicrobial peptides are potential alternatives to the traditional antibiotics and can be of great application to food and pharmaceutical industries; however, further studies are still needed to establish their drug-likeness and safety profiles.

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