Dataset Information

Large yellow croaker skin mucus proteome

ABSTRACT: Proteomics data demonstrate that skin mucus of the air-exposed large yellow croaker had a complex composition, with an unexpectedly high number of proteins (3,209), suggesting its multiple protective mechanisms possibly involved in antioxidant functions, oxygen transport, immune defence, and osmotic and ionic regulation. These results expand our knowledge of skin mucus secretion and function in fish, highlighting its importance in response to stress.

OTHER RELATED OMICS DATASETS IN: PRJNA245366PRJNA246784

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Larimichthys Crocea (large Yellow Croaker) (pseudosciaena Crocea)

TISSUE(S): Skin

SUBMITTER: fan ding

LAB HEAD: Xinhua Chen

PROVIDER: PXD001218 | Pride | 2015-04-17

REPOSITORIES: Pride

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Publications

Genome sequencing of the perciform fish Larimichthys crocea provides insights into molecular and genetic mechanisms of stress adaptation.

Ao Jingqun J Mu Yinnan Y Xiang Li-Xin LX Fan DingDing D Feng MingJi M Zhang Shicui S Shi Qiong Q Zhu Lv-Yun LY Li Ting T Ding Yang Y Nie Li L Li Qiuhua Q Dong Wei-Ren WR Jiang Liang L Sun Bing B Zhang XinHui X Li Mingyu M Zhang Hai-Qi HQ Xie ShangBo S Zhu YaBing Y Jiang XuanTing X Wang Xianhui X Mu Pengfei P Chen Wei W Yue Zhen Z Wang Zhuo Z Wang Jun J Shao Jian-Zhong JZ Chen Xinhua X

PLoS genetics 20150402 4

The large yellow croaker Larimichthys crocea (L. crocea) is one of the most economically important marine fish in China and East Asian countries. It also exhibits peculiar behavioral and physiological characteristics, especially sensitive to various environmental stresses, such as hypoxia and air exposure. These traits may render L. crocea a good model for investigating the response mechanisms to environmental stress. To understand the molecular and genetic mechanisms underlying the adaptation a ...[more]

PMID: 25835551

Similar Datasets

Project description:Antimicrobial peptides (AMPs) constitute a broad range of bioactive compounds in diverse organisms, including fish. They are effector molecules for the innate immune response, against pathogens, tissue damage and infections. Still, AMPs from African Catfish, Clarias gariepinus skin mucus are largely unexplored despite their possible therapeutic role in combating antimicrobial resistance. In this study, African Catfish Antimicrobial peptides (ACAPs) were identified from the skin mucus of African Catfish, C. gariepinus. Native peptides were extracted from fish mucus scrapings in 10% acetic acid (v/v) and ultra-filtered using 5kDa molecular cut-off membrane. The extract was purified using C18 Solid Phase Extraction. The antibacterial activity was determined using the Agar Well Diffusion method and broth-dilution method utilizing Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922). Thereafter, Sephadex G-25 gel filtration was further utilized in bio-guided isolation of the most active fractions prior to peptide identification using Orbitrap Fusion Lumos Tribrid Mass Spectrometry. The skin mucus extracted from African Catfish from all the three major lakes of Uganda exhibited antimicrobial activity on E. coli and S. aureus. Lake Albert’s C. gariepinus demonstrated the best activity with the lowest MIC of 2.84 µg/mL and 0.71 µg/mL on S. aureus and E. coli respectively. Sephadex G-25 peak I mass spectrometry analysis alongside in silico analysis revealed seven short peptides (11-15 amino acid residues) of high antimicrobial scores (0.561-0.905 units). In addition, these peptides had a low molecular weight (1005.57-1622.05 Da), and had percentage hydrophobicity above 54%. Up to four of these antimicrobial peptides demonstrated α-helix structure conformation, rendering them amphipathic. The ﬁndings of this study indicate that novel antimicrobial peptides can be sourced from the skin mucus of C. gariepinus. Such antimicrobial peptides are potential alternatives to the traditional antibiotics and can be of great application to food and pharmaceutical industries; however, further studies are still needed to establish their drug-likeness and safety profiles.

			Action	DRS
	L.crocea.MASCOT.search.dat	Other
	L.crocea.identify.summury.csv.gz	Csv
	L.crocea.mgf.zip	Mgf
	L.crocea.raw.zip	Raw