Dataset Information

Proteomics on visceral adipose tissue derived extracellular vesicles from Tsg101 knockout mice

ABSTRACT: The protein Tsg101 plays an integral role extracellular vesicle formation and secretion from cells. We developed a mouse model with Tsg101 knockdown (Tsg101dAd). Visceral adipose tissue (VAT) was harvested from Tsg101dAD and Tsg101flfl mice fed high-fat diet to induce obesity or matched diet as non-obese controls. VAT was ex vivo cultured for 48 hrs and its conditioned media was collected. Extracellular vesicles were isolated from conditioned media by ultracentrifugation. The aim of the project was to characterize extracellular vesicle protein cargo from obese and non-obese VAT.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Parsa Haque

LAB HEAD: Arianne Theiss

PROVIDER: PXD057436 | Pride | 2025-04-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20240202_SCP_HFD_Tsg101dAd_6496.d.zip	Other
	20240202_SCP_HFD_Tsg101dAd_6497.d.zip	Other
	20240202_SCP_HFD_Tsg101dAd_6498.d.zip	Other
	20240202_SCP_HFD_Tsg101dAd_6499.d.zip	Other
	20240202_SCP_HFD_Tsg101dAd_6500.d.zip	Other

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Publications

Obese Adipose Tissue Extracellular Vesicles Activate Mitochondrial Fatty Acid β-oxidation to Drive Colonic Stemness.

Haque Parsa S PS Goodman Desiree D Kuusivuori-Robinson Thor T Coughlan Christina C Delgado-Deida Yaritza Y Onyiah Joseph C JC Zempleni Janos J Theiss Arianne L AL

Cellular and molecular gastroenterology and hepatology 20250322 7

<h4>Background & aims</h4>Patients with obesity and mouse models of obesity exhibit abnormalities in intestinal epithelial cells, including enhanced stemness. Adipose tissue (AT) is the largest endocrine organ secreting cytokines, hormones, and extracellular vesicles (EVs). Here, we characterized EV protein cargo from obese and non-obese AT and demonstrate the role of obese adipose-derived EVs in enhancing colonic stemness.<h4>Methods</h4>EVs were isolated from visceral AT from mice fed high-fat ...[more]

PMID: 40122519

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