Survey of Tomato fields
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB28795 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:In this study, we obtain FoAPY1 transgenic tomato plants (2417-GFP) and control plants (GFP). All target tomato plants were identified by WB using anti-GFP antibody and all transgenic tomato plants has no differences in morphological and growth rate compared with wild type tomato plants. In order to detect the potential functional mechanism of FoAPY1 protein in the host plant, the proteomic analysis was performed to analyze differentially abundant proteins in two type tomato plants
2022-08-09 | PXD035875 | Pride
Project description:We use a human whole genome microarray to analyze the effects of nanosecond pulsed electric fields on Jurkat cells with the focus on early response genes to DNA damage. Keywords: nanosecond pulsed electric fields, jurkat cells, DNA damage
2006-02-01 | GSE4106 | GEO
Project description:Millet is a dangerous weed in Hungary. Lack of seed dormancy helps it to spread easily and be present at maize, wheat and other crop fields. Our previous report revealed the possibility that millet can also play a role as a virus reservoir. In that study we detected the presence of several viruses in millet using DAS ELISA. Because serological methods can only detect the presence of the investigated particular pathogens, we suspected that other, previously unknown viruses can also be present in this weed. To investigate this theory, we randomly sampled two locations and collected millets showing stunting, chlorosis, and striped leaves and investigated the presence of viruses using small RNA HTS as a diagnostic method. Our result confirmed the widespread presence of wheat streak mosaic virus at both locations. Moreover, barley yellow striate mosaic virus and barley virus G were also identified, which have not been described from Hungary before. As these viruses can cause severe diseases on wheat, their presence on a weed mean a potential infection risk. Our study indicates that the presence of millets on the fields needs a special control in order to prevent emergence of new diseases at crop fields.
2020-08-24 | GSE147185 | GEO
Project description:Tomato fruit ripening is associated with a dramatic increase in susceptibility to the fungal pathogen Botrytis cinerea, the causal agent of gray mold. Mature green fruit, prior to ripening, are largely resistant to B. cinerea, whereas red fruit, at the end of ripening, are susceptible to B. cinerea infection. We used microarrays to detail the gene expression changes that are induced by B. cinerea when tomato fruit at unripe and ripe stages are infected. Keywords: plant responses to pathogens
2009-05-22 | GSE14637 | GEO
Project description:Morphological and molecular characterization of tomato landraces from Lazio region in central Italy
2022-07-16 | PRJEB54712 | EVA
Project description:We used AML12 cells exposured with pulsed extremely low frequency weak magnetic fields for 4 msec at increasing frequency of 1, 2, 3, 4, 5, 6, 7, and 8 Hz for 1 sec each. The 1-to-8 Hz pulses over 8 sec were repeated indefinitely. Peak magnetic intensity was 100 mG. AML12 cells were incubated for 1 h under extremely low frequency weak magnetic fields.
2022-05-05 | GSE166811 | GEO
Project description:Purpose: The tomato psyllid, Bactericera cockerelli Šulc (Hemiptera: Triozidae), is a pest of tomato (Solanum lycopersicum) and potato (S. tuberosum) in the U.S. and vectors the disease-causing pathogen ‘Candidatus Liberibacter solanacearum’. Plants undergo physiological, transcriptomic, or epigenetic changes in order to mount a stronger, faster response against secondary challenges by previously perceived threats. This is called defense ‘priming’ and it likely has an impact on vectored disease transmission. Currently, it is still unknown whether or not psyllid infestation has any lasting consequences for tomato gene expression or defense. To characterize the genes potentially involved in tomato priming against psyllids, RNA was extracted from psyllid-primed and uninfested tomato (Moneymaker) leaves three weeks after infestation. Methods: RNA was extracted and sequenced from plants three weeks after psyllid infestation. Plants were either left alone (Control or C) or infested with psyllids (Primed or J1). Libraries were developed using the TruSeq RNA Library Prep Kit v2. Sequencing was performed on the Illumina PE HiSeq 2500 v4 platform. Processed sequences were uploaded to the CyVerse Discovery Environment computational infrastructure where bioinformatic analysis was performed using the Tuxedo Suite 2 workflow. Results: Illumina HiSeq sequencing of tomato cDNA libraries produced 132,428,443 total reads that met FastQC quality control criteria. 94.6% of all reads mapped to vSL3.0 of the S. lycopersicum genome. CuffDiff2 analysis identified 310 differentially expressed genes (DEGs) between control and psyllid-primed plants (q-value <0.01). Conclusions: A week-long infestation by a small number of B. cockerelli had lasting consequences for gene expression in tomato plants. Homologs of the DEGs were associated with 1) defense against abiotic and biotic stress, 2) growth and development, and 3) components of plant biology indirectly involved in plant growth and development such as homeostasis, transcription/translation, and molecular transport.
2019-04-11 | GSE129610 | GEO
Project description:Deep transcriptome sequencing of various tomato tissues. We describe the coupling of pyrosequencing technology with laser capture microdissection to characterize the transcriptomes of the five principal tissues of the pericarp from tomato fruits (outer and inner epidermal layers, collenchyma, parenchyma, and vascular tissues) at their maximal growth phase. NOTE: Not all samples from SRP004923 were imported in this accession.
2011-01-05 | E-MTAB-4765 | biostudies-arrayexpress
Project description:We used mRNA sequencing to study the effects of piperonylic acid treatment on the transcriptome of tomato plants (cv. Moneymaker) to gain insights in the molecular basis of piperonylic acid-induced disease resistance
2022-03-01 | GSE167407 | GEO
Project description:Pepino Mosaic Virus (PepMV) consists a major pathogenic threat in the greenhouses worldwide and has a devasting impact on tomato global production. PepMV belongs to the Potexvirus genus of the Flexiviridae family. PepMV has a viral RNA genome of approximately 6400 nucleotides long that contains five open reading frames (ORFs) and 4 major genotypes have been characterized. TomCr3, an indigenous virulent PepMV strain derived from the Chilean (CH2) genotype, was isolated in Crete, Greece and was used for the mechanical infection of Belladonna F1 hybrid tomato seedlings. Here, we present the results of a deep RNA-sequencing (RNA-seq) analysis performed to characterize the dynamic expression profile of tomato genes upon PepMV infection, including tomato transcription factors.
2022-05-24 | GSE203627 | GEO