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Immunologic pathways in protective versus maladaptive host responses to attenuated and pathogenic strains of Mycoplasma gallisepticum

ABSTRACT: Immunologic pathways in protective versus maladaptive host responses to attenuated and pathogenic strains of Mycoplasma gallisepticum

PROVIDER: PRJEB31142 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3147980_1.fastq.gz	Fastqsanger.gz
	ERR3147980_2.fastq.gz	Fastqsanger.gz
	ERR3147990_1.fastq.gz	Fastqsanger.gz
	ERR3147990_2.fastq.gz	Fastqsanger.gz
	ERR3148000_1.fastq.gz	Fastqsanger.gz

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Immunologic pathways in protective versus maladaptive host responses to attenuated and pathogenic strains of Mycoplasma gallisepticum

Project description:The goal of this study was to compare the host's response (chicken tracheal epithelium) to laboratory attenuated vaccine versus pathogenic strains of M. gallisepticum

2019-02-20 | E-MTAB-7675 | biostudies-arrayexpress

Immunologic Pathways in Protective versus Maladaptive Host Responses to Attenuated and Pathogenic Strains of Mycoplasma gallisepticum.

Project description:Mycoplasmas are small bacterial commensals or pathogens that commonly colonize host mucosal tissues and avoid rapid clearance, in part by stimulating inflammatory, immunopathogenic responses. We previously characterized a wide array of transcriptomic perturbations in avian host tracheal mucosae infected with virulent, immunopathologic Mycoplasma gallisepticum; however, mechanisms delineating these from protective responses, such as those induced upon vaccination, have not been thoroughly explored. In this study, host transcriptomic responses to two experimental M. gallisepticum vaccines were assessed during the first 2 days of infection. Relative to virulent infection, host metabolic and immune gene responses to both vaccines were greatly decreased, including early innate immune responses critical to disease development and subsequent adaptive immunity. These data specify host genes and potential mechanisms contributing to maladaptive versus beneficial host responses-information critical for design of vaccines efficacious in both limiting inflammation and enabling pathogen clearance.

| S-EPMC6386537 | biostudies-literature

Comparative Genomic Hybridizations of Mycoplasma gallisepticum Vaccine strains and strain Rlow

2009-12-24 | GSE19629 | GEO

Mycoplasma gallisepticum: Rlow vs. F

Project description:Mycoplasma gallisepticum transcriptome comparison between in vitro grown cultures of strains Rlow and F utilizing oligo DNA microarrays.

2010-01-07 | GSE19755 | GEO

Comparative Genomic Hybridizations of Mycoplasma gallisepticum Vaccine strains and strain Rlow

Project description:Genomic content of Vaccine strains were probed against the known sequence of the virulent strain Rlow of M. gallisepticum to identify divergent or absent genes in the attenuated strains. Genomic DNA was extracted from in vitro grown cultures of M. gallisepticum strains Rlow, F, ts-11, and 6/85 and three samples from each were selected for hybirdization to oligonucleotide microarrays using standard methods from the Bioprime CGH kit. All samples were labeled with Cy3 dye and scanned at a PMT gain of 700 using a GenePix 4000B scanner. Features are duplicated on the slides and data were averaged between duplicate features on each slide. Median signal intensities were averaged between samples of the same strain and each feature from each vaccine strain was compared to Rlow. Features exhibiting four-fold or less hybridization in the vaccine strains were considered divergent or absent.

2010-05-16 | E-GEOD-19629 | biostudies-arrayexpress

Transcription profiling of different state of Mycoplasma gallisepticum

Project description:Mycoplasma gallisepticum is a convenient model object for studying the regulation of transcription because it has a reduced genome, lack of cell wall and many metabolic pathways, and also easy to culture and non-pathogenic to humans. For rapid investigation of gene expression we developed microarray design including 3 366 probes for 678 genes. They included 665 protein coding sequences and 13 antisense RNAs from 816 genes and 17 ncRNAs present in Mycoplasma gallisepticum. This work was carried out transcriptomic profiling for different types of effects on the expression of genes of Mycoplasma gallisepticum: 1) genetic knock-out mutants; 2) cell culture exposed to sublethal concentrations of antibiotics; and 3) well-characterized heat stress effect. The study was performed on Agilent one-color microarray with custom design and random-T7 polymerase primer for cDNA synthesis. Using set of different probes for each gene or ncRNA allows to increase accuracy of gene expression quality.

2016-08-18 | GSE85777 | GEO

Mycoplasma gallisepticum: Rlow vs. F

Project description:Mycoplasma gallisepticum transcriptome comparison between in vitro grown cultures of strains Rlow and F utilizing oligo DNA microarrays. Two-condition experiment, Rlow vs. F strain cells. Biological replicates: 3. 1 technical replicate per biological replicate which includes a dye swap.

2010-04-22 | E-GEOD-19755 | biostudies-arrayexpress

Transcriptional Profiling of the Chicken Tracheal Response to Virulent Mycoplasma gallisepticum Strain Rlow

Project description:The goal of this study was to provide a global assessment of the host's response to M. gallisepticum over a 7-day time course

2017-07-14 | GSE101403 | GEO

Mycoplasma gallisepticum WhiA knockdown and overexpression

Project description:Proteomes of Mycoplasma gallisepticum strains with overexpression and knockdown of WhiA transcription factor. The overexpression was introduced on a transposon vector carrying M. gallisepticum whiA gene with strong constitutive promoter and strong SD sequence. The knockdown was made by CRISPRi. dCas9 protein and sgRNA against whiA gene were introduced on a transposon vector.

2021-06-25 | PXD026928 | Pride

Mycoplasma gallisepticum Rlow attached to MRC-5 cells microarray

Project description:The goal of this study was to identify genes important to the interaction of Mycoplasma gallisepticum with host cells in an in vitro system. An interaction time of one hour was chosen since it is less than a generation time and thus all changes can be attributed to transcriptional changes. A total of 60 transcripts were determined to be significantly up- or down-regulated under these conditions, including several hypothetical genes. Also several metabolism-related genes and ATP synthase genes were significantly down-regulated. Keywords: transcriptional response to host cells

2007-05-20 | GSE6717 | GEO

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