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Omics score: 0

Czech BP isolates -- recent vs historical

ABSTRACT: Comparative integrated omics analysis of historical and recent isolates of Bordetella pertussis

PROVIDER: PRJEB34096 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3489640.fastq.gz	Fastqsanger.gz
	ERR3489650.fastq.gz	Fastqsanger.gz
	ERR3489660.fastq.gz	Fastqsanger.gz
	ERR3489641.fastq.gz	Fastqsanger.gz
	ERR3489651.fastq.gz	Fastqsanger.gz

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COMPARATIVE OMICS ANALYSIS OF HISTORICAL AND RECENT ISOLATES OF BORDETELLA PERTUSSIS

Project description:Despite high vaccination coverage, pertussis is on the rise in many countries including Czech Republic. To better understand B. pertussis resurgence we compared the changes in genome structures between Czech vaccine and circulating strains and subsequently, we determined how these changes translated into global transcriptomic and proteomic profiles. The whole-genome sequencing revealed that both historical and recent isolates of B. pertussis display substantial variation in genome organization and cluster separately. The RNA-seq and LC-MS/MS analyses indicate that these variations translated into discretely separated transcriptomic and proteomic profiles. Compared to vaccine strains, recent isolates displayed increased expression of flagellar genes and decreased expression of polysaccharide capsule operon. Czech strains (Bp46, K10, Bp155, Bp318 and Bp6242)exhibited increased expression of T3SS and sulphate metabolism genes when compared to Tohama I. In spite of 50 years of vaccination the Czech vaccine strains (VS67, VS393 and VS401) differ from recent isolates to a lesser extent than from another vaccine strain Tohama I.

2021-09-08 | PXD015184 | Pride

Comparative genomic hybridization of Bordetella pertussis clinical isolates circulating in areas of intensive children vaccination and a reference strain

Project description:Genomic content of Bordetella pertussis clinical isolates circulating in areas of intensive children vaccination. 13 isolates and one reference strain of Bordetella pertussis used.

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Project description:Bordetella pertussis strain:BP 165 Genome sequencing and assembly

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Comparative genomic hybridization of 12 Bordetella holmesii strains compared to Bordetella pertussis

Project description:Comparative genome hybridization (CGH) of a sample of 12 Bordetella holmesii strains compared to Bordetella pertussis Tohama I

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BtcB (BP2265) is a T3SS chaperone of the CesT family in Bordetella pertussis

Project description:Bordetella pertussis is a Gram-negative, strictly human respiratory pathogen and the causative agent of whooping cough (pertussis). Similar to other Gram-negative pathogens, B. pertussis produces a functional type III secretion system, but its role in pathogenesis of B. pertussis is enigmatic and has not yet been elucidated. Here, we applied omics RNA-seq as well as LC-MS/MS techniques and co-immunoprecipitation method to identify and characterize the novel CesT family T3SS chaperone BP2265. Our results show that the chaperone BP2265 specifically interacts with the secreted T3SS anti-sigma factor BtrA. Moreover, in the absence of the chaperone, secretion but not production of BtrA and several early, intermediate, and late T3SS substrates is severely impaired. It appears that the role of BtrA in regulating T3SS is more complex and extends beyond its activity as an antagonist of the sigma factor BtrS. We propose to rename BP2265 as BtcB for the Bordetella type III chaperone of BtrA.

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Differential Proteomic Analysis of Bordetella pertussis OMV

Project description:Differential proteomic analysis of outer membrane vesicles from Bordetella pertussis (two strains: Bvg+ and Bvg-)

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Comparative genomic hybridization of 81 Bordetella pertussis strains

Project description:CGH analysis of 81 Bordetella pertussis strains

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