Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing of human iPSC-derived brain microvascular endothelial cells.

ABSTRACT: RNA sequencing of human iPSC-derived brain microvascular endothelial cells.

PROVIDER: PRJEB36836 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR3932730_1.fastq.gz	Fastqsanger.gz
	ERR3932730_2.fastq.gz	Fastqsanger.gz
	ERR3932731_1.fastq.gz	Fastqsanger.gz
	ERR3932731_2.fastq.gz	Fastqsanger.gz
	ERR3932726_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

mRNAseq of Huntington's disease and control patient iPSC-derived brain microvascular endothelial cells

Project description:Purpose: The goals of this study are to compare the transcriptomic profile (mRNA-seq) of HD and control patient iPSC-derived brain microvascular endotheial cells to identify alterations in gene expression. Methods: RNA were isolated from HD and control iPSC-derived brain microvascular endothelial cells. mRNAseq using Illumina TruSeq mRNA PolyA+ v2 lib prep and HiSeq 2500. Statistical difference in mRNA levels were calculated with subsequent GO and pathway analysis. Results: mRNAseq and statistical analysis revealed differentially expressed genes between HD and control iPSC-derived brain microvascular endothelial cells. Conclusions: Our study shows differentially expressed genes between HD and control iPSC-derived brain microvascular endothelial cells, and reveals gene networks that are relevant to the mechanism of HD pathogenesis.

2017-05-16 | GSE97100 | GEO

RNA-seq analysis of iPSC-derived brain microvascular endothelial cells following long-term cryopreservation

Project description:We report changes in gene expression from iPSC to iPSC-derived brain microvascular endothelial cells (dhBMECs) and from one-year cryopreservation of dhBMECs.

2020-06-08 | GSE151976 | GEO

RNA-seq expression data from Human iPSC-derived Brain Microvascular Endothelial-like Cells (iBMECs)

Project description:Comparison of Human iPSC-derived Brain Microvascular Endothelial-like Cells (iBMECs) grown in poly(dimethylsiloxane) tissue chips. Data contains RNA-seq profiles of iBMECs exposed to various levels of shear stress ranging from 0, 0.01, 0.5, and 2.4 dyn/cm2; as well as RNA-seq profiles of FACS sorted iBMECs cultured alone or with primary human astrocytes and pericytes or with iPSC-derived neural progenitor cells.

2019-06-05 | GSE129290 | GEO

SARS-CoV-2 causes dysfunction in human iPSC-derived brain microvascular endothelial like cells potentially by signal modulation

Project description:SARS-CoV-2 is considered to affect the central nervous system (CNS) by interacting with the blood–brain barrier (BBB), which is defined by tight junctions that seal paracellular gaps between brain microvascular endothelial like cells (BMECs). Although SARS-CoV-2 infection of BMECs has been reported, the mechanism has not been fully elucidated. Here, we investigated the mechanism using iPSC derived brain microvascular endothelial like cells (iPSC-BMELCs). We observed that iPSC-BMELCs were infected with SARS-CoV-2, which resulted in inflammatory responses. RNA-seq analysis revealed that SARS-CoV-2 modulated the expression of signal molecules in iPSC-BMELCs. These findings suggest that SARS-CoV-2 infection causes BBB dysfunction in humans.

2024-02-01 | GSE254309 | GEO

Induced pluripotent stem cell-derived cells model brain microvascular endothelial cell glucose metabolism

Project description:To investigate the validity of using iPSC-derived brain microvascular endothelial-like cells (BMEC) as a model of human BMEC metabolism, we ran RNA-sequencing on iPSC-derived BMEC and compared gene expression of glycolytic and TCA enzymes to publically avaliable primary BMEC datasets.

2022-08-22 | GSE211648 | GEO

RNA-sequencing of brain microvascular endothelial cell dysfunction in an isogenic juvenile iPSC model of Huntington’s disease

Project description:Huntington's disease (HD) is associated with dysfunction of the blood-brain barrier, including brain microvasscular endothelial cells (BMECs). We report changes in gene expression of induced pluripotent stem cells (iPSCs) and iPSC-derived brain microvascular endothelial cell-like cells (iBMECs) derived from an isogenic pair of iPSCs with either 180 (HD-corrected) or 18 (HD180) CAG repeats in the HTT gene.

2022-02-01 | GSE194416 | GEO

RNA sequencing of human iPSC-derived brain microvascular endothelial cells.

Project description:Induced pluripotent stem cells were differentiated to brain microvascular endothelial cells (BMECs) using previously published protocols with minor changes. RNA was collected from purified BMECs after barrier induction and submitted for sequencing.

2020-12-31 | E-MTAB-8802 | biostudies-arrayexpress

Effect of combinatorial transcription factor overexpression on iPSC-derived endothelial cells

Project description:We aimed to investigate how transcription factors alter brain-specific and zonation-identity of endothelial cells. We used a dox-inducible lentivirus to increase expression of specific combinations of transcription factors expressed by human brain endothelial cells in iPSC-derived endothelial cell (iEC) monolayers. Using SMART-seq we compared gene expression profiles and benchmarked gene expression to iPSC-derived brain microvascular endothelial-like cells (iBMECs).

2023-06-06 | GSE233694 | GEO

RNA-seq analysis of iPSC-derived brain microvascular endothelial cells following long-term cryopreservation

Project description:RNA-seq analysis of iPSC-derived brain microvascular endothelial cells following long-term cryopreservation

| PRJNA637914 | ENA

RNA-seq expression data from Human iPSC-derived Brain Microvascular Endothelial-like Cells (iBMECs)

Project description:RNA-seq expression data from Human iPSC-derived Brain Microvascular Endothelial-like Cells (iBMECs)

| PRJNA530756 | ENA