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RNA-seq analysis of iPSC-derived brain microvascular endothelial cells following long-term cryopreservation

ABSTRACT: RNA-seq analysis of iPSC-derived brain microvascular endothelial cells following long-term cryopreservation

PROVIDER: PRJNA637914 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11945714_1.fastq.gz	Fastqsanger.gz
	SRR11945714_2.fastq.gz	Fastqsanger.gz
	SRR11945715_1.fastq.gz	Fastqsanger.gz
	SRR11945715_2.fastq.gz	Fastqsanger.gz
	SRR11945716_1.fastq.gz	Fastqsanger.gz

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RNA-seq analysis of iPSC-derived brain microvascular endothelial cells following long-term cryopreservation

Project description:We report changes in gene expression from iPSC to iPSC-derived brain microvascular endothelial cells (dhBMECs) and from one-year cryopreservation of dhBMECs.

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mRNAseq of Huntington's disease and control patient iPSC-derived brain microvascular endothelial cells

Project description:Purpose: The goals of this study are to compare the transcriptomic profile (mRNA-seq) of HD and control patient iPSC-derived brain microvascular endotheial cells to identify alterations in gene expression. Methods: RNA were isolated from HD and control iPSC-derived brain microvascular endothelial cells. mRNAseq using Illumina TruSeq mRNA PolyA+ v2 lib prep and HiSeq 2500. Statistical difference in mRNA levels were calculated with subsequent GO and pathway analysis. Results: mRNAseq and statistical analysis revealed differentially expressed genes between HD and control iPSC-derived brain microvascular endothelial cells. Conclusions: Our study shows differentially expressed genes between HD and control iPSC-derived brain microvascular endothelial cells, and reveals gene networks that are relevant to the mechanism of HD pathogenesis.

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Genome-wide analysis of the effect of long-term cryopreservation on peripheral blood mononuclear cells

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SARS-CoV-2 causes dysfunction in human iPSC-derived brain microvascular endothelial like cells potentially by signal modulation

Project description:SARS-CoV-2 is considered to affect the central nervous system (CNS) by interacting with the blood–brain barrier (BBB), which is defined by tight junctions that seal paracellular gaps between brain microvascular endothelial like cells (BMECs). Although SARS-CoV-2 infection of BMECs has been reported, the mechanism has not been fully elucidated. Here, we investigated the mechanism using iPSC derived brain microvascular endothelial like cells (iPSC-BMELCs). We observed that iPSC-BMELCs were infected with SARS-CoV-2, which resulted in inflammatory responses. RNA-seq analysis revealed that SARS-CoV-2 modulated the expression of signal molecules in iPSC-BMELCs. These findings suggest that SARS-CoV-2 infection causes BBB dysfunction in humans.

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Cryopreservation effects on peripheral blood

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RNA sequencing of human iPSC-derived brain microvascular endothelial cells.

Project description:RNA sequencing of human iPSC-derived brain microvascular endothelial cells.

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Project description:Huntington's disease (HD) is associated with dysfunction of the blood-brain barrier, including brain microvasscular endothelial cells (BMECs). We report changes in gene expression of induced pluripotent stem cells (iPSCs) and iPSC-derived brain microvascular endothelial cell-like cells (iBMECs) derived from an isogenic pair of iPSCs with either 180 (HD-corrected) or 18 (HD180) CAG repeats in the HTT gene.

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