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IdAnoGamb

ABSTRACT: Anopheles gambiae

PROVIDER: PRJEB51754 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	ERR9356810_1.fastq.gz	Fastqsanger.gz
	ERR9356810_2.fastq.gz	Fastqsanger.gz
	ERR9356802_1.fastq.gz	Fastqsanger.gz
	ERR9356802_2.fastq.gz	Fastqsanger.gz

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Anopheles gambiae brain proteomic analysis

Project description:Proteomic analysis of Anopheles gambiae brain tissue after in-gel trypsin digestion. To gain insights into neurobiology of the Anopheles gambiae mosquito, we carried out a proteomic analysis of its brain using a comprehensive proteomic approach.

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Comparison of a resistant field population of Anopheles gambiae ss collected in Tiefora, Burkina Faso (2014) compared to a lab susceptible ss Anopheles gambiae Kisumu.

Project description:Comparison of a pyrethroid insecticides resistant field population of Anopheles gambiae ss collected in Tiefora, Burkina Faso (2014) compared to a lab susceptible ss Anopheles gambiae Kisumu.

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Project description:We characterize the epigenome of the human malaria vector Anopheles gambiae in midgut cells by mapping the distribution and levels of two post-translational histone modifications, H3K27ac and H3K27me3. These histone profiles were then correlated with levels of gene expression obtained by RNA-seq. ChIP-seq and RNA-seq were performed on adult female A. gambiae midguts. RNA-seq was performed on adult female A. gambiae salivary glands.

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2012-10-01 | E-GEOD-39269 | biostudies-arrayexpress

Anopheles gambiae

Project description:Anopheles gambiae

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Expression data from Anopheles gambiae females blood fed twice within a gonotrophic cycle

Project description:Anopheline mosquitoes frequently take multiple blood meals in a single gonotrophic cycle. In this study we determined patterns of gene expression in Anopheles gambiae females blood fed twice within the first gonotrophic cycle.

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Transcriptome analysis of virgin and mated Anopheles gambiae and Anopheles albimanus

Project description:We custom-built a bioinformatics pipeline to search for 20E-modifying enzymes in the accessory glands of Anopheles gambiae males, searching for ecdysteroid kinases (EcK), ecdysone oxidases (EO), and ecdysteroid-phosphate phosphatases (EPP). To this end, we generated RNAseq datasets of different An. gambiae tissues dissected from virgin and mated females and males, and produced similar datasets for Anopheles albimanus, a South American species that does not synthetize and transfer ecdysteroids during mating. These analyses led to the identification of one candidate EPP and two potential EcKs (EcK1 and EcK2), which we demonstrated are involved in the activity of a male-specific oxidized ecdysteroid (3D20E). We further determined that 3D20E is specifically produced by the An. gambiae male accessory glands and is transferred to females during copulation, where it triggers a series of post-mating responses.

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Expression data from the mosquito Anopheles gambiae midgut 3h after a protein meal with or without E. coli.

Project description:The Anopheles gambiae midgut harbors bacteria that proliferate upon a blood feed. We used microarrays to examine the midgut gene expression response at early stages (3hours) after an artifitial meal containing heat killed bacteria.

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Project description:In Sub-Saharan Africa, Anopheles gambiae Giles (Diptera: Culicidae) largely contributes to malaria transmission, in direct relation to environmental conditions influencing the vector ecology. Therefore, our study aimed to compare the proteomes of An. gambiae according to varying insecticide pressures associated to cotton crops also integrating different population origins from two climatic regions of Burkina Faso.

2020-03-09 | PXD016300 | Pride

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