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VISFATIN (NAMPT) AFFECTS GLOBAL GENE EXPRESSION IN PORCINE ANTERIOR PITUITARY CELLS DURING THE MID-LUTEAL PHASE OF THE OESTROUS CYCLE

ABSTRACT: The influence of visfatin on the porcine anterior pituitary cells on days 10 to 12 of the oestrous cycle

PROVIDER: PRJEB73811 | ENA |

REPOSITORIES: ENA

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VISFATIN (NAMPT) AFFECTS GLOBAL GENE EXPRESSION IN PORCINE ANTERIOR PITUITARY CELLS DURING THE MID-LUTEAL PHASE OF THE OESTROUS CYCLE

Project description:Pituitary belongs to a most important endocrine glands which takes part in the regulation of reproductive functions. The proper functioning of this intermediary between the central nervous system and the target tissues of the reproductive system ensures the proper course of the estrous cycle and affects the female's reproductive potential. It is believed that visfatin, a hormone belonging to the adipokine family, may be responsible for the control of reproductive functions in response to the actual metabolic state of female. Herein we verified hypothesis assuming the modulatory effect of visfatin on the anterior pituitary transcriptome during the mid-luteal phase of the oestrous cycle. We analyzed samples obtained from 5 individuals (n=5). After the enzymatic tissue digestion with the use collagenase V and pancreatin cells were divided for two groups: controls [without treatment] and visfatin (100 ng/mL) treated ones. In vitro cell cultures were conducted for 24 hours.

2024-05-01 | E-MTAB-13922 | biostudies-arrayexpress

The modulatory effect of visfatin (NAMPT) on the transcriptomic profile of porcine anterior pituitary cells during the peri-implantation period

Project description:Pregnancy is a unique and critical period in a female’s life during which the whole organism is mobilized and focused on ensuring the successful survival and development of the embryo. The main regulatory system responsible for the reproductive functions during both the oestrous cycle and pregnancy is the hypothalamic-pituitary-gonadal (HPG) axis. Herein we verified hypothesis assuming the modulatory effect of visfatin on the anterior pituitary transcriptome during the peri-implantation period. We analyzed samples obtained from 5 individuals (n=5). After the enzymatic tissue digestion with the use collagenase V and pancreatin cells were divided for two groups: controls [without treatment] and visfatin (100 ng/mL) treated ones. In vitro cell cultures were conducted for 24 hours.

2024-09-30 | E-MTAB-14347 | biostudies-arrayexpress

VISFATIN (NAMPT) AFFECTS GLOBAL GENE EXPRESSION IN PORCINE ANTERIOR PITUITARY CELLS DURING THE MID-LUTEAL PHASE OF THE OESTROUS CYCLE

Project description:VISFATIN (NAMPT) AFFECTS GLOBAL GENE EXPRESSION IN PORCINE ANTERIOR PITUITARY CELLS DURING THE MID-LUTEAL PHASE OF THE OESTROUS CYCLE

| PRJEB74058 | ENA

Visfatin (NAMPT) affects global gene expression in porcine anterior pituitary cells during the mid-luteal phase of the oestrous cycle.

Project description:BackgroundThe pituitary belongs to the most important endocrine glands involved in regulating reproductive functions. The proper functioning of this gland ensures the undisturbed course of the oestrous cycle and affects the female's reproductive potential. It is believed that visfatin, a hormone belonging to the adipokine family, may regulate reproductive functions in response to the female's metabolic state. Herein we verified the hypothesis that suggests a modulatory effect of visfatin on the anterior pituitary transcriptome during the mid-luteal phase of the oestrous cycle.ResultsRNA-seq analysis of the porcine anterior pituitary cells revealed changes in the expression of 202 genes (95 up-regulated and 107 down-regulated in the presence of visfatin, when compared to the non-treated controls), assigned to 318 gene ontology terms. We revealed changes in the frequency of alternative splicing events (235 cases), as well as long noncoding RNA expression (79 cases) in the presence of the adipokine. The identified genes were associated, among others, with reproductive system development, epithelial cell proliferation, positive regulation of cell development, gland morphogenesis and cell chemotaxis.ConclusionsThe obtained results indicate a modulatory influence of visfatin on the regulation of the porcine transcriptome and, in consequence, pituitary physiology during the mid-luteal phase of the oestrous cycle.

| S-EPMC11232246 | biostudies-literature

The modulatory effect of visfatin (NAMPT) on the transcriptomic profile of porcine anterior pituitary cells during the peri-implantation period

Project description:The modulatory effect of visfatin (NAMPT) on the transcriptomic profile of porcine anterior pituitary cells during the peri-implantation period

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Transcription profiling of rat anterior pituitary gland response to diethylstilbestrol

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The transcriptomic profile of porcine anterior pituitary cells is influenced by adiponectin

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Proteomic analysis of human anterior pituitary gland

Project description:The regulation of pituitary function via the hypothalamus and via intra-pituitary connections represents a complex system. Though hormones secreted from the pituitary glands have been well studied, overall information of proteins expressed in the pituitary glands is very limited. Protein expression profiling of normal pituitary tissue may lead to discovery of novel proteins playing an important role in the physiology of pituitary glands and can lead to better understanding of pituitary gland diseases. We aimed to carry out systematic proteomic profiling of adenohypophysis from human pituitary glands using high-resolution Fourier transform mass spectrometer. A total of 2,175 proteins were identified in this study of which, 105 proteins were identified for the first time as compared to high throughput proteomic-based studies from human pituitary glands. The comprehensive list of proteins identified in this study will facilitate the better understanding the role of this important gland in health and disease.

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The transcriptomic profile of porcine anterior pituitary cells is influenced by adiponectin

Project description:The transcriptomic profile of porcine anterior pituitary cells is influenced by adiponectin

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