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Adaptation to mistranslation in S. cerevisiae

ABSTRACT: Evolution of robustness to protein mistranslation by accelerated protein turnover

PROVIDER: PRJEB8951 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR833059_1.fastq.gz	Fastqsanger.gz
	ERR833059_2.fastq.gz	Fastqsanger.gz
	ERR833060_1.fastq.gz	Fastqsanger.gz
	ERR833060_2.fastq.gz	Fastqsanger.gz
	ERR833061_1.fastq.gz	Fastqsanger.gz

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Saccharomyces cerevisiae

Project description:Evolution of robustness to protein mistranslation by accelerated protein turnover

| PRJNA274784 | ENA

Evolution of robustness to protein mistranslation by accelerated protein turnover

2015-11-06 | GSE65718 | GEO

Evolution of robustness to protein mistranslation by accelerated protein turnover

Project description:To investigate how organisms mitigate the deleterious effects of mistranslation during evolution, a mutant tRNA was expressed in S. cerevisiae. The expression of Candida Ser-tRNACAG from a low copy plasmid in S. cerevisiae promoted mistranslation events by random incorporation of both serine and leucine at CUG codons. As mistranslation causes an overload of the protein quality pathways, it disrupts cellular protein homeostasis leading to a major fall in fitness. Laboratory evolutionary experiments were performed to study whether the fitness cost of mistranslation can be lowered. We also wanted to identify the cost-reduction strategy: reducing the frequencies of errors (mitigation), or increasing tolerance to errors (robustness), either by global or local activities. Gene expression was measured in the ancestor (non-evolved) lineage in two different situations: 1) carrying an empty vector and 2) expressing the mutant Ser-tRNACAG. Gene expression was also measured in three ambiguously evolved lineages (B3, D11, H9) in both situations (carrying an empty vector and expressing the mutant tRNA). Three independent experiments were performed for each lineage. Non-evolved strain with empty vector was used as control sample.

2015-11-06 | E-GEOD-65718 | biostudies-arrayexpress

Mistranslation accelerates the adaptation of Candida albicans to Fluconazole

Project description:The leucine CUG codon was reassigned to serine in the fungal pathogen Candida albicans. To clarify the biological role of this tuneable codon ambiguity on drug resistance, we evolved C. albicans strains that were engineered to mistranslate the CUG codon at constitutively elevated levels, in the presence and absence of the antifungal drug fluconazole. Elevated levels of mistranslation resulted in the rapid acquisition of resistance to fluconazole.

2016-10-27 | E-SYBR-13 | biostudies-arrayexpress

Biological effect of chronic mistranslation in mammalian cells

Project description:Analysis of HEK293 cells lines expressing mutant ribosomal protein RPS2 (human A226Y). RPS2 A226Y mutation has been shown to cause misreading and readthrough. Results provide insight into the response to chronic mistranslation in mammalian cells.

2019-09-24 | GSE115153 | GEO

Transcription profiling of S. cerevisiae in the 3 hours after induction of mRNA mistranslation

Project description:mRNA profiling analysis in S. cerevisiae cells in several time points (0 - 180 minutes) after mistranslation induction

2010-10-01 | E-MTAB-153 | biostudies-arrayexpress

Identifying the transcriptomic effects of tRNA-induced proline-to-serine mistranslation in Drosophila melanogaster

Project description:Mistranslation describes the misincorporation of an amino acid into a nascent polypeptide. Mistranslation has diverse effects on multicellular eukaryotes and is implicated in several human diseases. We introduced a mistranslating serine transfer RNA (tRNA) into Drosophila melanogaster that misincorporates serine at proline codons and found that it affected male and female flies differently. Here, we compare the transcriptomic response of male and female flies to mistranslation to identify cellular pathways that underlie this sex-specific response. Both males and females downregulated genes associated with metabolism in response to proline-to-serine mistranslation. Only males downregulated genes associated with developmental processes and response to negative stimuli such as infection, whereas only females downregulated aerobic respiration and ATP synthesis genes. Both sexes upregulated genes associated with gametogenesis but females upregulated cell cycle and DNA maintenance genes, suggesting that mistranslation may compromise genome integrity in females. This transcriptomic analysis advances our understanding of how males and females respond to mistranslation and has important implications for future studies that examine the influence of mistranslation on disease.

2024-05-11 | GSE256332 | GEO

Mistranslation accelerates the adaptation of Candida albicans to Fluconazole

Project description:Mistranslation accelerates the adaptation of Candida albicans to Fluconazole

| PRJEB8387 | ENA

Quantifying mistranslation from alanine tRNA anticodon variants in S. cerevisiae

Project description:Transfer RNAs (tRNAs) maintain translational fidelity through strict charging by their cognate aminoacyl-tRNA synthetase and codon:anticodon base pairing with the mRNA at the ribosome. Mistranslation occurs when an amino acid not specified by the genetic code is incorporated into a protein. Since alanyl-tRNA synthetase uniquely recognizes a G3:U70 base pair in alanine tRNAs and the anticodon plays no role in charging, alanine tRNA variants with anticodon mutations have the potential to mistranslate alanine. Our goal was to quantify mis-incorporation of alanine into proteins in Saccharomyces cerevisiae strains expressing one of 57 different alanine tRNA anticodon variants. Using mass spectrometry, we observed mistranslation for 45 of the variants when expressed on single-copy plasmids.

2023-10-02 | PXD038242 | Pride

Transcription profiling of yeast in response to mRNA mistranslation after 20 hours of induction

Project description:Transcriptome analysis in cells after 20 hours of mistranslation induction in relation to control cells at the same time point where mistranslation was not induced (without the inducible tRNA construction)

2010-10-01 | E-MTAB-165 | biostudies-arrayexpress

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