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Microbiota-dependent indole production is required for the development of collagen-induced arthritis

ABSTRACT: Microbiota-dependent indole production is required for the development of collagen-induced arthritis

PROVIDER: PRJNA1009050 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25757120_1.fastq.gz	Fastqsanger.gz
	SRR25757120_2.fastq.gz	Fastqsanger.gz
	SRR25757121_1.fastq.gz	Fastqsanger.gz
	SRR25757121_2.fastq.gz	Fastqsanger.gz
	SRR25757122_1.fastq.gz	Fastqsanger.gz

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Microbiota-dependent indole production is required for the development of collagen-induced arthritis

Project description:Altered tryptophan catabolism has been identified in inflammatory diseases like rheumatoid arthritis (RA) and spondyloarthritis (SpA), but the causal mechanisms linking tryptophan metabolites to disease are unknown. Using the collagen-induced arthritis (CIA) model we identify alterations in tryptophan metabolism, and specifically indole, that correlate with disease. We demonstrate that both bacteria and dietary tryptophan are required for disease, and indole supplementation is sufficient to induce disease in their absence. When mice with CIA on a low-tryptophan diet were supplemented with indole, we observed significant increases in serum IL-6, TNF, and IL-1β; splenic RORγt+CD4+ T cells and ex vivo collagen-stimulated IL-17 production; and a pattern of anti-collagen antibody isotype switching and glycosylation that corresponded with increased complement fixation. IL-23 neutralization reduced disease severity in indole-induced CIA. Finally, exposure of human colon lymphocytes to indole increased expression of genes involved in IL-17 signaling and plasma cell activation. Altogether, we propose a mechanism by which intestinal dysbiosis during inflammatory arthritis results in altered tryptophan catabolism, leading to indole stimulation of arthritis development. Blockade of indole generation may present a novel therapeutic pathway for RA and SpA.

2023-11-13 | GSE241655 | GEO

Fibroblast pyruvate carboxylase is required for collagen production in the tumor microenvironment

Project description:The aberrant production of collagen by fibroblasts is a hallmark of many solid tumors and can influence cancer progression. How fibroblasts produce collagen within tumor microenvironments which are nutrient-poor is not understood. Here, we show that pyruvate carboxylase (PC) activity increases in fibroblasts cultured in a low glutamine environment, and that PC is required for collagen production by tumor-associated fibroblasts in vivo.

2021-09-03 | GSE169588 | GEO

Fibroblast pyruvate carboxylase is required for collagen production in the tumor microenvironment

Project description:Fibroblast pyruvate carboxylase is required for collagen production in the tumor microenvironment

| PRJNA717053 | ENA

Transcription profiling of DBA/1J mouse with collagen induced arthritis

Project description:Time course of collagen-induced arthritis in DBA/1J male mice, synchronized on day 28 with 10ug LPS

2009-11-16 | E-TABM-661 | biostudies-arrayexpress

Microbiota-dependent indole production is required for the development of collagen-induced arthritis.

| S-EPMC10592798 | biostudies-literature

Project description:Microbiota-dependent indole production is required for the development of collagen-induced arthritis

| PRJNA1004672 | ENA

Collagen induced arthritis plasma proteomic analysis

Project description:The aim of the project is to analyze the plasma proteomic profile of an animal model of arthritis (Collagen Induced Arthritis; CIA) in response to different treatments. For this, plasma samples from mice under five different treatments were analyzed (N=6 per group): Vehicle, CIA, CIA + Δ9-THCA-A (20 mg / kg), CIA + Δ9-THCA-A (20 mg / kg) + T0070907 (5 mg / kg) and CIA + Δ9-THCA-A (20 mg / kg) + SR141716A (5 mg / kg).

2020-09-18 | PXD015274 | Pride

Transcriptomic analysis of synovial cells derived from collagen-induced arthritis mice

Project description:The destruction of bone and cartilage results in a loss of joint functionality, critically impairing the quality of life in arthritis patients. Synovial fibroblasts (SFs) critically contribute to the pathogenesis of rheumatoid arthritis (RA) by acquiring either a pro-inflammatory or tissue-destructive phenotype. To explore the molecular mechanisms underlying the pathogenic fibroblast phenotype in arthritis, we performed single-cell RNA sequencing (scRNA-seq) on the synovial cells which were isolated from collagen-induced arthritis (CIA) mice.

2022-07-06 | GSE192504 | GEO

Collagen induced arthritis in dba/1j mice associates with oxylipin changes in plasma

Project description:Oxylipins play important roles in various biological processes and are considered as mediators of inflammation for a wide range of diseases such as rheumatoid arthritis (RA). The purpose of this research was to study differences in oxylipin levels between a widely used collagen-induced arthritis (CIA) mice model and healthy control (Ctrl) mice. DBA/1J male mice (age: 6-7 weeks) were selected and randomly divided into two groups, viz. a CIA- and a Ctrl group. The CIA mice were injected intraperitoneal (i.p.) with the joint cartilage component collagen type II (CII) and an adjuvant injection of lipopolysaccharide (LPS). Oxylipin metabolites were extracted from plasma for each individual sample using solid phase extraction (SPE) and were detected with high performance liquid chromatography/tandem mass spectrometry (HPLC-ESI-MS/MS), using dynamic multiple reaction monitoring (dMRM). Both univariate and multivariate statistical analysis was applied. The results in univariate student's t-test revealed 10 significantly up- or down-regulated oxylipins in CIA mice, which were supplemented by another 6 additional oxylipins, contributing to group clustering upon multivariate analysis. The dysregulation of these oxylipins revealed the presence of ROS-generated oxylipins and an increase of inflammation in CIA mice. The results also suggested that the Collagen-induced arthritis might associate with dysregulation of apoptosis, possibly inhibited by activated NF- κ B because of insufficient PPAR-γ ligands.

2015-11-04 | MTBLS243 | MetaboLights

HIF2α-regulated genes in small intestine epithelial cells from collagen induced arthritis model

Project description:HIF2α works as an important transcription factor to induce target genes mediating cell proliferation, meabolism and inflammation. To gain new insights into HIF2α modulated genes, we used high-throughput sequencing to analyze potential HIF2a regulated genes in small intestine cells from collagen induced arthritis model mice.

2024-05-31 | GSE176266 | GEO

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