Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1063580 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The invasive Halyomorpha halys Stål, the Brown Marmorated Stink Bug (BMSB), and the native Nezara viridula L., the Southern Green Stink Bugs (SGSB), are widely distributed in Europe, even if the date of introduction and their diet differ. Saliva of Hemipteran pests plays an essential role in the interaction between insects and their host plants. Several aphid salivary proteomes have been identified and found to differ according to the species, while no comparative investigation between phytophagous stink bugs has been performed yet. Here we analyzed the salivary proteins from two bugs, BMSB and SGSB, using LC-MS/MS. A total of 238 and 305 proteins were identified from dissected salivary glands from BMSB and SGSB respectively. Among these, a large majority was found in both species. In comparison with salivary proteome from other Hemiptera, the most striking feature of the salivary gland proteomes from SGSB and BMSB is the similar protein functions patterns. Some of the proteins are speculated to be dependent of the feeding strategies, playing a significant role in plant-insect interactions. Our results provide a framework for future research to elucidate the molecular basis of differential impact of piercing-sucking insects on host plants.
2019-02-25 | PXD011920 | Pride
Project description:The invasive Halyomorpha halys Stål, the Brown Marmorated Stink Bug (BMSB), and the native Nezara viridula L., the Southern Green Stink Bugs (SGSB), are widely distributed in Europe, even if the date of introduction and their diet differ. Saliva of Hemipteran pests plays an essential role in the interaction between insects and their host plants. Several aphid salivary proteomes have been identified and found to differ according to the species, while no comparative investigation between phytophagous stink bugs has been performed yet. Here we analyzed the salivary proteins from two bugs, BMSB and SGSB, using LC-MS/MS. A total of 238 and 305 proteins were identified from dissected salivary glands from BMSB and SGSB respectively. Among these, a large majority was found in both species. In comparison with salivary proteome from other Hemiptera, the most striking feature of the salivary gland proteomes from SGSB and BMSB is the similar protein functions patterns. Some of the proteins are speculated to be dependent of the feeding strategies, playing a significant role in plant-insect interactions. Our results provide a framework for future research to elucidate the molecular basis of differential impact of piercing-sucking insects on host plants.
2019-02-25 | PXD011976 | Pride
Project description:Predatory bugs capture prey by injecting venom from their salivary glands using specialized stylets. Understanding venom function has been impeded by a scarcity of knowledge of their venom composition. We therefore examined the proteinaceous components of the salivary venom of the predatory stink bug Arma chinensis (Hemiptera: Pentatomidae). Using gland extracts and venoms from 5th-instar nymphs or adult females, we performed shotgun proteomics combined with venom gland transcriptomics. We found that the venom of A. chinensis comprised a complex suite of over a hundred individual proteins, including oxidoreductases, transferases, hydrolases, ligases, protease inhibitors, and recognition, transport and binding proteins. Besides the uncharacterized proteins, hydrolases such as venom serine proteases, cathepsins, phospholipase A2, phosphatases, nucleases, alpha-amylases, and chitinases constitute the most abundant protein families. However, salivary proteins shared by and unique to other predatory heteropterans were not detected in A. chinensis venom. Injection of the proteinaceous (> 3 kDa) venom fraction of A. chinensis gland extracts or venom into its prey, the larvae of the Oriental armyworm Mythimna separata (Walker, 1865), revealed insecticidal activity against lepidopterans. Our data expands the knowledge of heteropteran salivary proteins and suggests predatory asopine bugs as a novel source for bioinsecticides.
2023-07-20 | PXD040272 | Pride
Project description:We analyzed ORC2 ChIP-Seq from hand dissected salivary glands of wandering third instar larvae from OrR or SuUR Drosophila. Goals were to ascertain the difference in binding profile between salivary glands expressing and not expressing the Supressor of UnderReplication protein. One replicate is included for each of OrR (WT) or SuUR salivary glands.
2011-10-18 | E-GEOD-31899 | biostudies-arrayexpress
Project description:The salivary glands often become damaged in individuals receiving radiotherapy for head and neck cancer, resulting in chronic dry mouth. This leads to detrimental effects on their health and quality of life, for which there is no regenerative therapy. Macrophages are the predominant immune cell in the salivary glands and are attractive therapeutic targets due to their unrivalled capacity to drive tissue repair. Yet, the nature and role of macrophages in salivary gland homeostasis, and how they may contribute to tissue repair following injury is not well understood. Here, using scRNAseq we profile the transcriptomes of adult mouse salivary gland macrophages at steady state (D0) and at days 3 and 28 after targeted irradiation injury to define the transcriptional profiles of macrophages during homeostasis, acute injury and regeneration. We sorted epithelial cells (CD326+), endothelial cells (CD31+) and macrophages (CD45+CD11b+F4/80+) and sequenced their transcriptomes using 10x Genomics Single Cell 3' (v3.1). For each sample equal numbers of cells from 2 mice were pooled.
2023-10-01 | E-MTAB-13374 | biostudies-arrayexpress
Project description:Studying the salivary glands potential role during intestinal infections,using advantage from the ingenious GAL4/UAS-system available in the fly Epithelial immunity is a very simple but nevertheless indispensable type of immune response. Amongst all epithelial tissues, the intestine and the airways are outstanding, because they provide huge surface areas, where colonization or invasion of potential pathogens has to be obviated. The intestine is of special interest, because it has to hold the balance between tolerance and immunity, to protect the own microbial flora. An essential part of the intestinal tract has been completely overlooked, the salivary glands. They are the gatekeepers of the intestinal system, being essential for various aspects of the intestineâs immunity. Using the bipartite Gal4/UAS expression system, it is possible to activate the immune system ectopically in different organs of the fly. Activation of the IMD-pathway in the salivary glands is possible; because a very specific driver line is available that directs expression of any gene of interest into the salivary glands only. Flies, where the IMD-pathway in the salivary glands has been activated have a very distinct phenotype. They show a smaller body length as well as a smaller salivary gland length than the parental animals Microarray data analysis showed that 457 genes were upregulated and 578 genes downregulated. Interestingly, the sets of regulated genes show only a very small overlap with the canonical set of Drosophila immune genes. Other physiological scenarios such as autophagic cell death are apparently also not activated upon IMD-pathway activation. Among the regulated genes, those that code for signaling associated protease activity are significantly modulated. This holds especially true for presenilin and the signal peptide peptidase. The comparison of the transcriptional events induced following IMD-activation in the trachea and the salivary glands shows also only a small overlap, indicating that the general IMD-activated core transcriptome is rather small. In conclusion, the salivary glands may a very good tool to study the physiological role of selected genes that are of importance for human health such as the Alzheimerâs disease related presenilin gene in a functional environment. Ectopic activiation of the Immune deficiency pathway in the larval salivary glands using the Gal4/UAS-system of Brand and Perrimon. The activation was induced by the overexpression of the pattern recognition receptor PGRP-LCx using a driver line which was specific for the salivary glands.In general four replicates were performed including dye-swaps in two-colour arrays.
2010-05-05 | E-GEOD-20938 | biostudies-arrayexpress
Project description:ChIP was performed to identify regions of gDNA bound by H3K27me3 in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that H3K27me3 binds differently in under-replicated in SuUR mutant third instar salivary glands.
2011-10-18 | GSE31897 | GEO
Project description:We analyzed ORC2 ChIP-Seq from hand dissected salivary glands of wandering third instar larvae from OrR or SuUR Drosophila. Goals were to ascertain the difference in binding profile between salivary glands expressing and not expressing the Supressor of UnderReplication protein.
2011-10-18 | GSE31899 | GEO