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ABSTRACT: Transcriptomes of pentatomid salivary glands

PROVIDER: PRJNA838574 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19221691.fastq.gz	Fastqsanger.gz
	SRR19221692.fastq.gz	Fastqsanger.gz
	SRR19221693.fastq.gz	Fastqsanger.gz
	SRR19221694.fastq.gz	Fastqsanger.gz
	SRR19221695.fastq.gz	Fastqsanger.gz

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ChIP-Seq of ORC2 bound to third instar salivary gland DNA in WT and mutant Drosophila, analyzed by Illumina sequencing

Project description:We analyzed ORC2 ChIP-Seq from hand dissected salivary glands of wandering third instar larvae from OrR or SuUR Drosophila. Goals were to ascertain the difference in binding profile between salivary glands expressing and not expressing the Supressor of UnderReplication protein. One replicate is included for each of OrR (WT) or SuUR salivary glands.

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Project description:The salivary glands often become damaged in individuals receiving radiotherapy for head and neck cancer, resulting in chronic dry mouth. This leads to detrimental effects on their health and quality of life, for which there is no regenerative therapy. Macrophages are the predominant immune cell in the salivary glands and are attractive therapeutic targets due to their unrivalled capacity to drive tissue repair. Yet, the nature and role of macrophages in salivary gland homeostasis, and how they may contribute to tissue repair following injury is not well understood. Here, using scRNAseq we profile the transcriptomes of adult mouse salivary gland macrophages at steady state (D0) and at days 3 and 28 after targeted irradiation injury to define the transcriptional profiles of macrophages during homeostasis, acute injury and regeneration. We sorted epithelial cells (CD326+), endothelial cells (CD31+) and macrophages (CD45+CD11b+F4/80+) and sequenced their transcriptomes using 10x Genomics Single Cell 3' (v3.1). For each sample equal numbers of cells from 2 mice were pooled.

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ChIP with anti-H3K27me3 to compare binding in salivary glands of WT and SuUR Drosophila

Project description:ChIP was performed to identify regions of gDNA bound by H3K27me3 in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that H3K27me3 binds differently in under-replicated in SuUR mutant third instar salivary glands.

2011-10-18 | GSE31897 | GEO

ChIP-Seq of ORC2 bound to third instar salivary gland DNA in WT and mutant Drosophila, analyzed by Illumina sequencing

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Next Generation Sequencing Facilitates Quantitative Analysis of Murine Salivary Gland Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular functions. The goal of this study is to compare NGS-derived salivary gland transcriptome profilings (RNA-seq) to better understand the molecular nature of the physiological differences in adult murine salivary glands. Methods: Major murine salivary gland mRNA profiles were generated by deep sequencing, in triplicate, using Illumina HiSeq 2000. The sequence reads that passed quality filters were analyzed at the gene level with STAR followed by Cufflinks. In vivo NaCl reabsorption measurements were performed for validation. Results: Using an optimized data analysis workflow, we mapped about 15 million sequence reads per sample to the mouse genome (build mm10) and identified 1991 genes that were differentially expressed across three major salivary glands. RNA-seq data provided valuable insights into the nature of the functional differences among the major salivary glands Conclusions: Our study represents the first detailed analysis of murine salivary gland transcriptomes, with biologic replicates, generated by RNA-seq technology. Our results confirm functions of many genes, identified using genetically modified mice. We conclude that RNA-seq-based transcriptome characterization would offer a comprehensive and sensitive evaluation of the gene expression.

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RNAPolII ChIP to find differences between third instar salivary glands of WT and SuUR

Project description:ChIP was performed to identify regions of gDNA bound by RNA polymerase II in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that RNAPolII does not localize to regions that are under-replicated in SuUR mutant third instar salivary glands.

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