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High-throughput exploration of single-cell transcriptional alterations associated with remote fear memory formation

ABSTRACT: High-throughput exploration of single-cell transcriptional alterations associated with remote fear memory formation

PROVIDER: PRJNA1070279 | ENA |

REPOSITORIES: ENA

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High-throughput exploration of single-cell transcriptional alterations associated with remote fear memory formation

Project description:Leveraging SPEED in tandem with snRNA-Seq, we uncover molecular identities of distinct neural subtypes, concurrent 5hmC alterations at active enhancers and gene bodies, and dynamic gene expression associated with remote memory formation. This comprehensive analysis encompasses several thousand cells from the cerebral and cerebellar cortex of home-caged (HC) and fear-conditioned (FC) mice.

2025-01-01 | GSE254380 | GEO

Strain differences in responsiveness to chronic restraint stress affect remote contextual fear memory and blood transcriptomics

Project description:Fear memory regulation is thought to be similar between humans and animals, and the role of stress in altering remote memory is little explored. Since individual variation in stress reactivity exists, should stress alter remote memory, invividuals with differing stress-reactivity would affect it to various degree. We evaluate this question using two strains of rats with differing stress-reactivity. The Fisher 344 (F344) strain is known to have active coping style and represent normal stress-reactivity, while the Wistar–Kyoto (WKY) rat shows passive coping strategies and heightened stress-reactivity. Male animals were exposed contextual fear conditioning (CFC) and four weeks later, chronic restraint stress (CRS) or no stress (NS) was administered for two weeks. Remote memory, immediate stress response to a second CFC, and reinstated fear memory was measured. Both recent and reinstated fear memory were greater in F344s, regardless of the stress status, In contrast, remote memory was attenuated in F344 only, concurring with their increased immediate stress responsive behavior after CRS. To find if this strain-specific response to CRS can be mirrored by transcriptomic changes in the blood, RNA sequencing was carried out. Overlapping differentially expressed genes (DEGs) between NS vs. CRS in the blood of F344 and WKY suggest a convergence of stress-related molecular mechanisms, independent of stress-reactivity. In contrast, DEGs unique to the F344 and the WKY stress responses are divergent in their functionality and networks, beyond that of strain differences in their non-stressed state. These unique DEGs than could be implicated as biomarkers stress-reactivity.

2020-10-01 | GSE148151 | GEO

Epigenetic priming of memory updating during reconsolidation to attenuate remote fear memories

Project description:Remembrances of traumata range among the most enduring forms of memories. Despite the elevated lifetime prevalence of anxiety disorders, effective strategies to attenuate long-term traumatic memories are scarce. The most efficacious treatments to diminish recent (i.e., day-old) traumata capitalize on memory updating mechanisms during reconsolidation that are initiated upon memory recall. Here, we show that in mice successful reconsolidation-updating paradigms for recent memories fail to attenuate remote (i.e., month-old) ones. We find that whereas recent memory recall induces a limited period of hippocampal neuroplasticity mediated, in part, by S-nitrosylation of HDAC2 and histone acetylation, such plasticity is absent for remote memories. However, by using an HDAC2-targeting inhibitor (HDACi) during reconsolidation, even remote memories can be persistently attenuated. This intervention epigenetically primes the expression of neuroplasticity-related genes as revealed by whole genome RNA sequencing, which is accompanied by higher metabolic, synaptic and structural plasticity. Thus, applying HDACis during memory reconsolidation might constitute a treatment option for remote traumata. 3 biological replicates per group were analyzed. The material analyzed was whole hippocampi from one brain hemisphere, from which total RNA was extracted.

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Specific loss of remote fear memory by knockdown of a neuron-specific nucleosome remodeling factor BAF53b in the amygdala

Project description:Despite recent advances, molecular events and mechanisms involved in long-lasting memory formation are unclear. Here, we report a critical role for nucleosome remodeling factor BAF53b in mice. We observed an increase of BAF53b expression beyond 24 h after fear conditioning in the lateral amygdala, which was crucial for memory retention at remote time. RNA-seq analyses and behavioral rescue experiments identified fibroblast growth factor-1 (FGF1) as a candidate downstream effector.

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Epigenetic priming of memory updating during reconsolidation to attenuate remote fear memories

2014-01-04 | GSE53794 | GEO

Single-cell 5-hydroxymethyl cytosine dynamics during remote memory formation

Project description:We introduce the Split-Pool based Epigenetic Decoding (SPEED) method, a combinatorial barcoding strategy enhancing the throughput of single-cell 5-hydroxymethyl cytosine (5hmC) or 5-methylcytosine (5mC) profiling. SPEED enables simultaneous, unbiased examination of 5hmC traits across distinct brain regions, surpassing limitations of individual cell scrutiny. Using SPEED we unveil dynamic 5hmC patterns linked to remote memory formation in the brain.

2025-01-01 | GSE254382 | GEO

Strain differences in responsiveness to chronic restraint stress affect remote contextual fear memory and blood transcriptomics

Project description:Strain differences in responsiveness to chronic restraint stress affect remote contextual fear memory and blood transcriptomics

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Sex differences in the role of K63 polyubiquitination in the amygdala during fear memory formation

Project description:This project examined sex differences in the role of K63 polyubiquitination in the amygdala during context fear memory formation in male and female rats.

2023-05-18 | PXD036369 | Pride

Sex differences in the role of protein sumoylation in the amygdala during fear memory formation

Project description:This project examined sex differences in the role of protein sumoylation in the amygdala during context fear memory formation in male and female rats.

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Sex differences in the role of protein degradation in the amygdala during fear memory formation

Project description:This project examined sex differences in the role of degradation-specific protein polyubiquitination in the amygdala during context fear memory formation in male and female rats.

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