Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Se Deficiency Exacerbates Hyperoxia-Induced Lung Injury in Newborn C3H/HeN Mice

ABSTRACT: Se Deficiency Exacerbates Hyperoxia-Induced Lung Injury in Newborn C3H/HeN Mice

PROVIDER: PRJNA1076259 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR27962200_1.fastq.gz	Fastqsanger.gz
	SRR27962200_2.fastq.gz	Fastqsanger.gz
	SRR27962201_1.fastq.gz	Fastqsanger.gz
	SRR27962201_2.fastq.gz	Fastqsanger.gz
	SRR27962202_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

Se Deficiency Exacerbates Hyperoxia-Induced Lung Injury in Newborn C3H/HeN Mice

Project description:Extremely preterm infants are often treated with supraphysiological oxygen which contributes to the development of bronchopulmonary dysplasia (BPD). These same infants exhibit compromised antioxidant capacities due in part to selenium (Se) deficiency. The present study was designed to develop a perinatal Se deficiency mouse model, identify the effects of newborn hyperoxia exposure, and explore alternative pathways affected by Se deficiency (SeD) that would contribute to impaired lung development. Se deficient breeding pairs were generated, once pups were born, they were exposed to room air or 85% O2 for 14 d. Survival, antioxidant and Nrf2-regulated protein expression, and RNA seq analyses were performed. Greater than 40% mortality was observed in Se deficient (SeD), hyperoxia exposed pups. Surviving SeD pups had greater lung growth deficits than Se sufficient (SeS) pups exposed to hyperoxia. Gpx2 and 4 protein and Gpx activity were significantly decreased in SeD pups. Nrf2-regulated proteins, NQO1 and Gclc were increased in the setting of Se deficiency and hyperoxia exposure. RNA seq revealed significant decreases in the Wnt/-catenin and Notch pathways. Se is a biologically relevant modulator of perinatal lung development and antioxidant responses, especially in the context of hyperoxia exposure. RNA seq implicates pathways essential for normal lung development are dysregulated by Se deficiency.

2024-05-01 | GSE255744 | GEO

WGBS-seq, CUTANDRUN, ATAC-seq of urothelial stem cells isolated from Juvenile Naïve C3H/HeN mice

Project description:To study epigenetic changes in mouse bladder urothelial cells with different infection histories, urothelial stem cells (USCs) isolated from C3H/HeN mice with different infection histories (Adult Naive, Resolved, and Sensitized) were used to perform WGBS , ATAC, and CUT&RUN for comparison.

2023-08-21 | GSE212094 | GEO

Differences in nucleus accumbens transcriptome are associated with high motivation for palatable reward in C3H/hen male mice exposed to maternal separation [hypothalamus]

Project description:The aim of this study was to determine the impact of early life stress on the motivation for a palatable nutritional reward in two mouse strains. Our data showed that MS associated with unpredictable chronic mild stress in lactating dams exacerbated motivation for a palatable food reward (sweetened milk) in both males and females C3H/HeN mice. Interestingly, no effect of MS was reported on motivation in C57Bl/6J mice strain. The transcriptomic analysis revealed that exacerbated motivation in MS C3H/HeN male mice was associated with marked changes in gene expressions in the NAc, whereas no significant changes were reported in PFC or hypothalamus.

2023-07-24 | GSE222777 | GEO

Differences in nucleus accumbens transcriptome are associated with high motivation for palatable reward in C3H/hen male mice exposed to maternal separation [nucleus accumbens]

2023-07-24 | GSE222779 | GEO

Differences in nucleus accumbens transcriptome are associated with high motivation for palatable reward in C3H/hen male mice exposed to maternal separation [medial prefrontal cortex]

2023-07-24 | GSE222780 | GEO

Mus musculus strain:C3H/HeN | isolate:Naive Resolved Sensitized

Project description:RNA-seq, ATAC-seq, WGBS, and CUT&RUN of urothelial cells isolated from Naive, Resolved, and Sensitized C3H/HeN mice

| PRJNA705407 | ENA

C3H-HeH

Project description:C3H-HeH

| PRJEB10874 | ENA

Citrobacter rodentium triggers exaggerated mucosal responses in mice developing severe intestinal disease

Project description:Citrobacter rodentium is commonly used to elucidate mucosal responses to infection in mice developing mild disease (e.g. C57BL/6), while little is known about host responses to infection in mice developing severe disease (e.g. C3H/HeN). We report that the phyla Bacteroidetes is a minor component of the tissue-associated microbiome in uninfected C3H/HeN mice. Following infection, C. rodentium rapidly and uniformly colonises the C3H/HeN colonic mucosa, which coincides with downregulation of proteins involved in the TCA cycle and oxidative phosphorylation in intestinal epithelial cells (IECs). In contrast, we observed upregulation of DNA replication and DNA damage repair processes, as well as cholesterol biogenesis, import and export, nutritional immunity, IL-22 and INFg responses, and expression of NLRP3, in IECs. Moreover, C. rodentium triggers a staggered cell proliferation response from 3 days post infection, which correlated with a higher abundance of SLC5A9 and reduced abundance of the IEC differentiation markers SLC26A3 and CA4. Uniquely, C. rodentium triggers differential secretion of gel-forming mucins, with the number of goblet cells filled with acidic and neutral mucins dramatically increasing and decreasing, respectively. Together, these results show that despite vigorous responses, C3H/HeN mice succumb to C. rodentium infection, possibly as a result of excessive and disordered mucosal responses.

2020-01-21 | PXD012846 | Pride

Aurothioglucose Enhances Pro-Angiogenic Pathway Activation in Lungs from Room Air and Hyperoxia-exposed Newborn Mice

Project description:Acute neonatal muring hyperoxia exposure to evaluate the impact of the TXNRD1 inhibitor aurothioglucose on pulmonary response and gene expression.

2020-07-15 | GSE145912 | GEO

Gene regulation in the hyperoxia mouse retina

Project description:PURPOSE: Hyperoxia is toxic to photoreceptors, and this toxicity may be important in the progress of retinal dystrophies. This microarray study examines gene expression induced in the C57BL/6J mouse retina by hyperoxia over the 14-day period during which photoreceptors first resist, then succumb to, hyperoxia. METHODS: Young adult C57BL/6J mice were exposed to hyperoxia (75% oxygen) for up to 14 days. On day 0 (control), day 3, day 7, and day 14, retinal RNA was extracted and processed on Affymetrix GeneChip Mouse Genome 430 2.0 arrays. Microarray data were analyzed using GCOS Version 1.4 and GeneSpring Version 7.3.1. RESULTS: The overall numbers of hyperoxia-regulated genes increased monotonically with exposure. Within that increase, however, a distinctive temporal pattern was apparent. At 3 days exposure, there was prominent upregulation of genes associated with neuroprotection. By day 14, these early-responsive genes were downregulated, and genes related to cell death were strongly expressed. At day 7, the regulation of these genes was mixed, indicating a possible transition period from stability at day 3 to degeneration at day 14. CONCLUSIONS: Microarray analysis of the response of the retina to prolonged hyperoxia demonstrated a temporal pattern involving early neuroprotection and later cell death, and provided insight into the mechanisms involved in the two phases of response. As hyperoxia is a consistent feature of the late stages of photoreceptor degenerations, understanding the mechanisms of oxygen toxicity may be important therapeutically. 4 timepoints in total, 0d (control), 3d, 7d and 14d and a replicate per timepoint. Total of 8 chips.

2010-08-05 | E-GEOD-23437 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data