Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA107675 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Skeletal muscle has been identified as a secretory organ. We hypothesized that IL-6, a cytokine secreted from skeletal muscle during exercise, could induce production of other secreted factors in skeletal muscle. Keywords: Human skeletal muscle time course response to IL-6 infusion IL-6 was infused for 3 h into healthy young males (n=7) and muscle biopsies obtained at time points 0, 3 and 6 h in these individuals. Affymetrix microarray analysis of gene expression profiling in muscle were carried out on 3 randomly chosen subjects.
2008-05-27 | E-GEOD-10685 | biostudies-arrayexpress
Project description:Effect of insulin infusion on human skeletal muscle
| PRJNA98187 | ENA
Project description:Skeletal muscle has been identified as a secretory organ. We hypothesized that IL-6, a cytokine secreted from skeletal muscle during exercise, could induce production of other secreted factors in skeletal muscle. Keywords: Human skeletal muscle time course response to IL-6 infusion
2008-05-28 | GSE10685 | GEO
Project description:In this experiment we have analyzed the effect of TGFbeta-1 incubation (3h of incubation time) on the gene expression profiles of human primary cultured skeletal muscle cells, both in growing myoblasts or in differentiated muscle fibres (after 10-days of differentiation), obtained from skeletal muscle biopsies from a 2-months old healthy donor and a 5-years old DMD (Duchenne Muscular Dystrophy) patient. All human skeletal muscle biopsies were obtained by surgery from calf muscles with informed consent and approval of the Human Use Committee of Vall d'Hebron University Hospital (Barcelona, Spain). Human myoblasts were cultured on 6 cm plates until reached 70-80% of confluence and then, were incubated for 3h in the presence or in absence of TGF-?1 (2.5 ng/ml). Skeletal muscle fibres were obtained by growing human myoblasts on 6 cm plates until reached 70-80% of confluence and then, cells were induced to differentiate by deprivation of growth factors in the culture medium. After 10 days of differentiation, skeletal muscle fibers were incubated for 3h in the presence or in absence of TGFbeta-1 (2.5 ng/ml). In all cases, immediately after TGFbeta-1 treatment the RNA was extracted and used for Affymetrix microarrays analysis using the U133 2.0 microarray genechips.
2016-08-07 | E-MEXP-2937 | biostudies-arrayexpress
Project description:To identify insulin responsive genes in humans, in the first protocol, skeletal muscle biopsies from six non-diabetic subjects were obtained before and after a two-hour of hyperinsulinaemic (infusion rate 40 mU/m2/min) euglycemic clamp. A variable infusion of glucose (180 g/l) enriched with tritiated glucose (100 μCi/500 ml) maintained euglycemia during insulin infusion, with monitoring of plasma glucose concentration every 5 to 10 min during the basal and clamp periods using an automated glucose oxidation method (Glucose Analyzer 2, Beckman Instruments, Fullerton, CA). In the second protocol, skeletal muscle biopsies from six non-diabetic subjects were obtained before and after a 3-hour hyperinsulinemic (infusion rate 40 mU/m2/min) euglycemic clamp in order to increase the effects of insulin on gene expression. A variable infusion of glucose (180 g/l) was used to maintain euglycemia during insulin infusion with monitoring of plasma glucose concentration every 5 to 10 min using an automated glucose oxidation method (Glucose Analyzer 2, Beckman Instruments, Fullerton, CA). Keywords: dose response The muscle biopsies were obtained from the vastus lateralis muscle under local anesthesia before and after hyperinsulinaemic (infusion rate 40 mU/m2/min) euglycemic clamp
2007-03-01 | E-GEOD-7146 | biostudies-arrayexpress
Project description:Insulin resistance, the defective regulation of glucose metabolism by insulin, increases the risk of type 2 diabetes and cardiovascular disease. It is not yet known how insulin resistance remodels signalling networks in human tissues. Here, we define the signalling architecture of insulin resistance in skeletal muscle. We measured phenotypes and phosphoproteomes of insulin resistant and insulin sensitive subjects as they responded to exercise and an insulin infusion, quantifying more than 26,000 phosphosites in 114 skeletal muscle biopsies. Incorporating a temporal component to personalised phosphoproteomics identified signalling linked with the range of insulin sensitivity across the participants.
2024-11-22 | PXD032948 | Pride
Project description:We examined global mRNA expression using cDNA microarrays in skeletal muscle of humans before, and 3h and 48h after 300 maximal eccentric contractions. Keywords: Time course Healthy, non-trained university-aged subjects performed 300 single leg maximal eccentric contractions. Skeletal muscle biopsies were taken from the vastus lateralis before, 3h and 48h after the exercise bout. Total RNA was extracted, amplified, reverse transcribed, and cDNA was analyzed on a custom made cDNA microarray. Four subjects were analyzed, and samples were not pooled between subjects (i.e. individual microarrays were used for baseline vs. 3H and baseline vs. 48h for EACH subject; repeated measures design).
2006-04-15 | E-GEOD-4249 | biostudies-arrayexpress
Project description:Skeletal muscle biopsies before and after hyperinsulinemic-euglycemic clamp
| PRJNA105677 | ENA
Project description:Expression data from skeletal muscle biopsies of healthy adults
| PRJNA213559 | ENA