Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of DRG-derived cell culture

ABSTRACT: RNA-seq of DRG-derived cell culture

PROVIDER: PRJNA1087425 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR28338148.fastq.gz	Fastqsanger.gz
	SRR28338149.fastq.gz	Fastqsanger.gz
	SRR28338150.fastq.gz	Fastqsanger.gz
	SRR28338151.fastq.gz	Fastqsanger.gz
	SRR28338152.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

RNA-seq of DRG-derived cell culture

Project description:We performed mRNA-seq to characterize cultured dorsal root ganglia (DRG) cells from adult mice. Our data is based on ~25 million reads per sample in six independent RNA preparations. The transcript per millions (TPM) gene count distribution was homogenous between individual cultures. We analyzed the transcriptome for marker genes of different cell types present in the DRG. Expression profiles pointed to a sensory progenitor-like cell type originating from peripheral glial cells of the adult DRG. Such progenitor cells are interesting for reprogramming.

2024-06-20 | GSE261550 | GEO

Mouse DRG RNA-Seq

Project description:Mouse DRG RNA-Seq

| PRJNA895420 | ENA

DRG-CSC co-culture tissue engineered model, CSCs monoculture model and DRG monoculture model

Project description:In this study, we constructed a tissue-engineered DRG-CSC (corneal stromal cells) co-cultured model (DS model), a CSC monoculture model (S model), and a DRG monoculture model (D model). Chicken DRGs were extracted from E7-E10 chicken embryo. Human CSCs were extracted in stromal lenticules from small incision lenticule extraction (SMILE). Transcriptional profiling of DRG in the DS model (DS-D), DRG in the D model(D-D), CSCs in the DS model (DS-S), CSCs in the S model (S-S) was analyzed. After 7 days of culture, RNA of each group was extracted.

2021-06-29 | GSE179020 | GEO

DRG-CSC co-culture tissue engineered model, CSCs monoculture model and DRG monoculture model

Project description:DRG-CSC co-culture tissue engineered model, CSCs monoculture model and DRG monoculture model

| PRJNA741931 | ENA

Genome-wide transcriptome analysis of neuron-enriched DRG cultures exposed to Wnt3a

Project description:To test the potential of canonical Wnt signalling to modulate nociception via transcriptional regulation in dorsal root ganglion (DRG) neurons, we performed a genome-wide transcriptome analysis of neuron-enriched DRG cultures treated with Wnt3a or vehicle for 6 hours. Total RNA obtained from neuron-enriched mouse DRG culture subjected to Wnt3a treatment for 6 hours was compared to a matched control (vehicle-treated) DRG culture.

2014-07-07 | E-GEOD-56880 | biostudies-arrayexpress

RNA-Seq of regenerating DRG neurons

Project description:To identify isoform differential expression underlying peripheral nerve regeneration we performed RNA-Sequencing on DRG neurons after axotomy.

2017-07-12 | GSE59547 | GEO

Mouse DRG RNA-Seq

Project description:Multiple myeloma (MM) is a neoplasia of B plasma cells that often induces bone pain. However, the mechanisms underlying myeloma-induced bone (MIBP) pain are mostly unknown. Using a localized MM mouse model, we investigated MM induced gene expression changes in the dorsal root ganglia (DRG) innervating the MM-bearing bone and found alterations in pathways associated with cell cycle, immune response and neuronal signalling. The MM transcriptional signature was consistent with metastatic MM infiltration to the DRG, a never-before described feature of the disease that we further demonstrated histologically. In the DRG, MM cells caused loss of vascularization and neuronal injury, which may contribute to late-stage MIBP.

2023-06-26 | GSE216802 | GEO

RNA-seq to identify differentially expressed mRNAs after cKO of Ybx1 in DRG [RNA-seq]

Project description:To identify the target mRNAs of Ybx1 in mouse DRG, we first performed RNAseq to screen differentially expressed mRNAs after knockout of Ybx1. We generated Wnt1-cre+/-;Ybx1fl/fl cKO mice to specifically knockout Ybx1 in DRG. Then we collected E13.5 DRG from cKO mice and litermate Ybx1fl/fl control mice. After RNA extraction and library construction, RNA sequcencing was performed. Finally, we identified 1638 differentially expressed mRNAs. This study provides a gene list which shows mRNA that can be regulated by Ybx1 in mouse DRG.

2024-11-15 | GSE255394 | GEO

RNA-Seq of DRG and dorsal cord after nerve injury

Project description:Genes are up and down regualted in DRG and spinal dorsal cord after peripheral nerve injury WT male adult with sciatic and femoral nerve transection 7 days, RNA was purified from ipilateral or contralateral L4-L6 DRGs or lumbar spinal dorsal cords

2015-12-10 | E-GEOD-66619 | biostudies-arrayexpress

Npr2-mediated cGMP signalling modulates S-palmitoylation and controls growth cone morphology of DRG neurons

Project description:A cGMP signalling cascade composed of the ligand C-type natriuretic peptide (CNP), the natriuretic peptide receptor 2 (Npr2) and the cGMP-dependent kinase Iα (cGKI) is implicated by growth cone splitting in the bifurcation of afferents from dorsal root ganglia (DRG), cranial sensory ganglia (CSG) and mesencephalic trigeminal neurons (MTN). To get further mechanistic insights into the process of axon bifurcation we applied different cell culture approaches to decipher downstream activities of cGKI in somatosensory growth cones. We demonstrate that CNP induces an enlargement of DRG growth cones via cGKI which can be blocked or stimulated by pharmacological reagents that interfere with S-palmitoylation. cGKI colocalizes primarily in the central domain of the growth cone with the palmitoylome and vesicular structures including the endoplasmic reticulum, early endosomes, lysosomes and in the soma in the Golgi apparatus. Consistently, an acyl-biotin-exchange chemistry-based screen indicated that cGMP signalling regulates S-palmitoylation of a restricted pool of proteins in the DRG-derived cell line F11.

2018-10-18 | PXD010326 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data