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ABSTRACT: RNA-seq of ring glands after CG33557 and Tap RNAi in Drosophila prothoracic glands

PROVIDER: PRJNA1091181 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28428137_1.fastq.gz	Fastqsanger.gz
	SRR28428137_2.fastq.gz	Fastqsanger.gz
	SRR28428138_1.fastq.gz	Fastqsanger.gz
	SRR28428138_2.fastq.gz	Fastqsanger.gz
	SRR28428139_1.fastq.gz	Fastqsanger.gz

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Project description:In Drosophila melanogaster larvae the ring gland is a control center that orchestrates major developmental transitions. It is a composite organ, consisting of the prothoracic gland, the corpus allatum and the corpora cardiaca, each of which synthesizes and secretes a different hormone. Until now, the ring gland’s broader developmental roles beyond endocrine secretion have not been explored. RNA sequencing and analysis of a new transcriptome resource from D. melanogaster wandering third instar larval ring glands has provided a fascinating insight into the diversity of developmental signalling in this organ. We have found strong enrichment of expression of two gene pathways not previously associated with the ring gland: immune response and fatty acid metabolism. We have also uncovered strong expression for many uncharacterized genes. Additionally, RNA interference against ring gland-enriched cytochrome p450s Cyp6u1 and Cyp6g2 produced a lethal ecdysone deficiency and a juvenile hormone deficiency respectively, flagging a critical role for these genes in hormone synthesis. This transcriptome provides a valuable new resource for investigation of roles played by the ring gland in governing insect development.

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