Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA109195 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:This SuperSeries is composed of the following subset Series:; GSE11256: KCl depolarization-regulated genes in mouse cortical neurons; GSE11258: Npas4-regulated genes in mouse hippocampal neurons Experiment Overall Design: Refer to individual Series
2008-10-26 | E-GEOD-11261 | biostudies-arrayexpress
Project description:We performed a DNA microarray experiment to identify activity-regulated genes that are misregulated in the absence of Npas4. Experiment Overall Design: We infected mouse hippocampal neurons with lentivirus expressing Npas4-RNAi or control-RNAi @ 3 DIV and depolarized the neurons @ 8 DIV with 50 mM of KCl for 0, 1, 3 or 6 hours. Neurons were lysed, mRNA isolated and hybridized to Affymetrix arrays. Data were collected from 3 independent experiments.
2008-10-26 | E-GEOD-11258 | biostudies-arrayexpress
Project description:Identification of synaptic activity-regulated genes in primary mouse hippocampal neurons
| PRJNA357078 | ENA
Project description:Identification of activity-induced Npas4-regulated genes in cortical inhibitory and excitatory neurons (array)
| PRJNA240171 | ENA
Project description:RNAi profiling of mouse hippocampal neurons infected with lentivirus expressing Npas4-RNAi or control-RNAi to study Npas4-regulated genes
2020-04-03 | E-GEOD-11258 | ExpressionAtlas
Project description:KCl depolarization-regulated genes in mouse cortical neurons
| PRJNA109193 | ENA
Project description:mouse hippocampal neurons HT22 pSTAT3 Genome sequencing
| PRJNA757795 | ENA
Project description:To identify the activity-induced gene expression programs in inhibitory and excitatory neurons, we analyzed RNA extracted from cultured E14 mouse MGE- and CTX-derived neurons (DIV 10) after these cultures were membrane-depolarized for 0, 1 and 6 hrs with 55mM extracellular KCl. To identify the gene programs regulated in these cells by the activity-induced early-response transcription factor Npas4, we repeated the same experiment in the MGE- and CTX-cultures lacking Npas4 (Npas4-KO). Littermate mouse E14 MGE- or CTX-derived neurons (WT or KO for Npas4) were cultured for 9 days, quieted overnight with TTX and AP-5 and then membrane-depolarized for 0, 1 or 6 hours by raising the extracellular KCl-concentration to 55mM. RNA was then extracted and analyzed using Affymetrix GeneChip Mouse Expression Set 430 2.0 microarray platform.
2015-06-04 | E-GEOD-55588 | biostudies-arrayexpress
Project description:Introgressed variants from other species can be an important source of genetic variation because they may arise rapidly, can include multiple mutations on a single haplotype, and have often been pretested by selection in the species of origin. Although introgressed alleles are generally deleterious, several studies have reported introgression as the source of adaptive alleles-including the rodenticide-resistant variant of Vkorc1 that introgressed from Mus spretus into European populations of Mus musculus domesticus. Here, we conducted bidirectional genome scans to characterize introgressed regions into one wild population of M. spretus from Spain and three wild populations of M. m. domesticus from France, Germany, and Iran. Despite the fact that these species show considerable intrinsic postzygotic reproductive isolation, introgression was observed in all individuals, including in the M. musculus reference genome (GRCm38). Mus spretus individuals had a greater proportion of introgression compared with M. m. domesticus, and within M. m. domesticus, the proportion of introgression decreased with geographic distance from the area of sympatry. Introgression was observed on all autosomes for both species, but not on the X-chromosome in M. m. domesticus, consistent with known X-linked hybrid sterility and inviability genes that have been mapped to the M. spretus X-chromosome. Tract lengths were generally short with a few outliers of up to 2.7 Mb. Interestingly, the longest introgressed tracts were in olfactory receptor regions, and introgressed tracts were significantly enriched for olfactory receptor genes in both species, suggesting that introgression may be a source of functional novelty even between species with high barriers to gene flow.
| S-EPMC8784167 | biostudies-literature
Project description:miRNA profiling array and analysis in mouse hippocampal neurons
| PRJNA151617 | ENA