Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1092856 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Drought is a major limiting constraint to faba bean production worldwide, including Tunisia. However, molecular mechanisms underlying faba bean responses to drought stress are not well understood. In this context, transcriptome analysis by RNA-seq was performed to investigate drought-related genes and construct a network of faba bean drought stress response and tolerance. De novo assembly of the transcriptome generated 26,728 differentially expressed genes (DEGs). Of these, 13,920 were up-regulated and 12,808 down-regulated in faba bean drought-stressed leaves. Moreover, a total of 10,800 simple sequence repeats (SSRs) and 2130 transcription factors involved in major metabolic pathways including abscisic acid (ABA)-dependent and -independent signaling pathway were identified. GO, KOG and KEGG enrichment analyses revealed that these DEGs were involved in several important processes including photosynthesis, flavonoid biosynthesis, response to stimulus and abiotic stress, reactive oxygen species (ROS) scavengers, signal transduction, biosynthesis of secondary metabolites and transporters, suggesting the involvement of these important pathways in faba bean response to water deficit. Various stress proteins such as late embryogenesis abundant proteins (LEA), dehydrins (DHNs) and heat shock proteins (HSPs) have been identified and their expression was robustly upregulated in drought-stressed leaves, indicating their key contribution to drought response and adaptation by conferring protection and providing stability to faba bean plant cellular processes under water deficit. The reliability of the RNA-seq results was confirmed by the analysis of 10 randomly selected genes using qRT-PCR. Taken together, these findings help advancing our knowledge and can guide breeding programs aimed at improving the tolerance of faba bean to drought stress.
2025-03-31 | GSE292422 | GEO
Project description:Drought is one of the most crucial environmental stresses limiting faba bean growth and productivity in the Mediterranean region. In order to explore how the faba bean plant responds to drought stress, a physiological and proteomic analysis was performed in leaf tissue. All physiological parameters were affected by drought. The physiological mechanism underlying the response of faba bean leaves to drought was therefore attributed to the alleviation of oxidative stress via the accumulation of organic solutes such as proline and to the synergistic action of the antioxidant enzyme system (CAT, SOD, APX and GPOX). Proteomic analysis identified 2000 proteins from faba bean leaves, of which were 81 differentially expressed. Of those, 45 were upregulated and 36 were downregulated under drought treatment. GO and KEGG enrichments indicated differentially abundant proteins (DAPs) associated with photosynthesis, antioxidants/detoxifying enzymes, molecular chaperones, biosynthesis of amino acids and secondary metabolites, signal transduction, energy and carbohydrate metabolism and metabolic enzymes. The current results provide evidence for a complex synergetic pathway, in which ROS detoxification mechanisms and photoprotection constituted the major aspect of drought tolerance in faba bean leaves. These results offer a foundational basis regarding the molecular mechanism involved in drought resistance within the faba bean species
2024-04-30 | PXD041759 | Pride
Project description:In the present study, a faba bean protein isolate (wBPC) with almost ~80 % crude protein produced by a wet process was investigated in feeds for Atlantic salmon in seawater. Four dietary treatments were tested including one treatment with high inclusion of fishmeal (400 g kg-1, named FM) and three treatments with low fishmeal (216 g kg-1) and increasing inclusions of faba bean protein concentrate (0, 70 and 140 g kg-1) substituting soy protein concentrate (236, 125 and 45 g kg-1), named SPC, BPC7 and BPC14 respectively.
2016-04-20 | E-MTAB-4661 | biostudies-arrayexpress
Project description:High-quality sources of protein for the formulation of feeds of carnivorous fish species such as Atlantic salmon are currently being sought. In an earlier screening trial we evaluated for the first time in Atlantic salmon (Salmo salar) the applicability of air-classified faba bean (Vicia faba) protein concentrate (BPC) inclusions in combination with soy protein concentrate (SPC) and fishmeal (FM) using parr as a model. Based on the results in parr in freshwater, the present study tested the hypothesis that BPC can effectively replace SPC as a dietary protein source in post-smolt Atlantic salmon in seawater. Herein we compare three dietary treatments, including BPC0 (no BPC), BPC20 (20% BPC) and BPC40 (40% BPC). Full details on diet formulation are available in the publication.
2015-08-19 | E-MTAB-3835 | biostudies-arrayexpress
Project description:iTRAQ-Based Proteomics Analysis Revealed Proteins Involved in Drought Response During the Germination Stage in Faba Bean
2023-11-17 | PXD047083 |