Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1093236 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR28522800.fastq.gz | Fastqsanger.gz | |||
| SRR28522801.fastq.gz | Fastqsanger.gz | |||
| SRR28522802.fastq.gz | Fastqsanger.gz | |||
| SRR28522803.fastq.gz | Fastqsanger.gz | |||
| SRR28522804.fastq.gz | Fastqsanger.gz |
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Project description:Black soldier fly larvae meal (BSFL) from Hermetia illucens is a promising alternative protein source in diets for farmed fish. The larvae can efficiently convert low-value organic material into high quality protein in a production cycle with low arable land and freshwater inputs. A few recent studies have shown that BSFL is a suitable protein source for Atlantic salmon (Salmo salar) in smaller controlled experiments. However, industry-relevant field trials conducted under large scale near-commercial conditions over a longer period are lacking. In this study, a feeding trial was performed to evaluate the impact of BSFL on growth performance and health of Atlantic salmon during the grow out phase in seawater, in a commercial site in Vestland county, Norway. A total of 320,000 post-smolt Atlantic salmon were distributed into six duplicate sea cages and fed one of three diets (commercial-like control diet and two test diets partially replacing the protein content of the control diet with 4 % and 8 % defatted BSFL meal) for 21 weeks, until a relevant commercial slaughter size of 4.5-5.0 kg was reached. Health parameters were assessed including histology of the distal intestine (DI), in addition to DI microbiota identification (by 16s rRNA-seq) and salmon RNA-seq of DI and head kidney (HK). The results showed that the inclusion of BSFL meal supported growth performance and had no adverse effect on gut health. The beta diversity of the distal intestine microbiota and the relative abundance of families Lactobacillaceae and the chitinolytic Bacillaceae increased in the fish fed the BSFL diets. Additionally, no histopathological changes were attributable to BSFL meal intake. Results from RNA-seq in DI revealed that BSFL inclusion modulates metabolic processes associated with lipids, the response to estrogens, the activity of immune receptors (to chemokines), phagocytosis and extracellular vesicles. Based on these results, black soldier fly larvae meal is a suitable alternative protein ingredient in inclusions of up to at least 8 % for Atlantic salmon under industrial fish farming conditions.
2024-08-16 | GSE241320 | GEO
Project description:Contamination of food products with mycotoxins such as aflatoxin B1 (AFB1) poses a severe risk to human health. Larvae of the black soldier fly (BSFL), Hermetia illucens (Diptera: Stratiomyidae), can successfully metabolize AFB1 without any negative consequences on their survival or growth. However, the underlying mechanisms that allow BSFL to metabolize AFB1 are unknown. In this study, five-day-old BSFL were fed with either a control or an AFB1-spiked (20 µg/kg) diet to elucidate the underlying mechanisms. Larval samples were collected at three timepoints (6 h, 24 h, and 72 h) and subjected to RNA-Seq analysis to determine gene expression patterns. Provision of an AFB1-spiked diet resulted in an up-regulation of 357 and a down-regulation of 929 unique genes. Upregulated genes include multiple genes involved in AFB1 metabolism in other (insect) species. Downregulated genes were generally involved in the insects' growth, development, and immunity. BSFL possesses a diverse genetic arsenal that encodes for enzymes capable of metabolizing AFB1 without trade-offs on larval survival.
2024-06-18 | GSE263746 | GEO
Project description:Although the basic anatomical sub-divisions of the larval mosquito gut were established several decades ago, information regarding their exact physiological roles is rather scarce. Several studies have reported differences between larval gut compartments in various morphological and physiological aspects. Unfortunately, the fragmentary and incomplete nature of this information makes it hard to establish clear links to the specific and/or unique physiological roles of each gut region. In this study, we attempted to identify transcripts significantly enriched in each gut section of the 4th instar Anopheles gambiae larva. We took advantage of a commercially-available microarray platform containing ~14,000 different An. gambiae transcripts (Affymetrix GeneChip® Plasmodium/Anopheles array, Affymetrix Inc., Santa Clara, CA). Based on the information obtained, we discuss the putative physiological roles of each major compartment of the larval gut, and identify potential new targets for larval control strategies. Experiment Overall Design: We attempted to identify transcripts significantly enriched in each gut section of the 4th instar Anopheles gambiae larva. Signal intensities of transcripts from each gut section were compared to those of a matching group of whole 4th instar larvae using t-tests. Transcripts showing an intensity value two-fold or higher (p < 0.05) in each gut section as compared to whole larvae were considered to be significantly enriched.
2008-11-06 | E-GEOD-7149 | biostudies-arrayexpress
Project description:The larvae of black soldier fly (BSF) Hermetia illucens (Diptera: Stratiomyidae), has demonstrated ability in the efficient bioconversion of organic waste into a sustainable source of food and feed, but fundamental biology remains to be discovered to exploit their full biodegradative potential. Herein, LC-MS/MS was used to assess the efficiency of eight differing extraction protocols to build foundational knowledge regarding the proteome landscape of both BSF larvae body and gut. No specific protocol was superior in capturing the BSF body and gut proteome, but each yielded complementary information to improve BSF proteome coverage. Protocol-specific functional annotation using protein level information has shown that the selection of extraction buffer can affect protein detection and their associated functional classes within the measured BSF larval gut proteome. Metaproteome analysis on BSF larvae gut has uncovered the prevalence of two bacterial phyla: actinobacteria and proteobacteria. We envisage that comparing a range of extraction protocols and investigating the proteome from the BSF body and gut separately will expand the fundamental knowledge of the BSF proteome and thereby provide translational opportunities for future research to enhance their efficiency for waste degradation and contribution to the circular economy.
2023-03-11 | PXD037977 | Pride
Project description:Although the basic anatomical sub-divisions of the larval mosquito gut were established several decades ago, information regarding their exact physiological roles is rather scarce. Several studies have reported differences between larval gut compartments in various morphological and physiological aspects. Unfortunately, the fragmentary and incomplete nature of this information makes it hard to establish clear links to the specific and/or unique physiological roles of each gut region. In this study, we attempted to identify transcripts significantly enriched in each gut section of the 4th instar Anopheles gambiae larva. We took advantage of a commercially-available microarray platform containing ~14,000 different An. gambiae transcripts (Affymetrix GeneChip® Plasmodium/Anopheles array, Affymetrix Inc., Santa Clara, CA). Based on the information obtained, we discuss the putative physiological roles of each major compartment of the larval gut, and identify potential new targets for larval control strategies. Keywords: tissue comparison
2007-10-01 | GSE7149 | GEO
Project description:An evolved strain (ECA5) presented two successive yields of bioconversion of higher alcohols to acetate esters, while its parental strain (EC1118) had a constant yield through the fermentation. Transcriptomic analysis was performed during wine fermentation in SM330 containing 8mg/L phytosterols, at 35 g/l and at 70 g/l of CO2 released. For ECA5, this corresponds to a sample before and one after the change of bioconversion yield. This analysis helped us to understand the different management of lipid source by the evolved strain, which is probably linked to a greater availability in acetyl-CoA. Two strains (EC1118 and ECA5) were compared during wine fermentation at 2 released CO2 time point (35g/L and 70g/L). Each condition is in triplicat.
2016-06-29 | E-GEOD-68354 | biostudies-arrayexpress
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Project description:Expression of mutant lamins in human muscle causes muscular dystrophy. We have generated a drosophila model that expresses mutant lamins, modeled after those that cause disease in humans. We used affymetrix microarrays to determine the global changes in gene expression caused by mutant lamins. Dissected 3rd instar larval body wall from transgenic Drosophila expressing either a full length wildtype Lamin C, an N-terminal Lamin C truncation of the first 42 amino acids or a single amino acid substitution in Lamin C at position 489 from a G residue to a V residue under control of the Gal4-UAS. RNA was extracted from 3rd instar larval body wall expressing these mutant Lamin C transgenes and microarray analysis using Affymetrix microarrays.
2015-04-16 | E-GEOD-49959 | biostudies-arrayexpress
Project description:Manipulation of host and parasite microbiotas: Survival strategies during chronic nematode infection
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