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Transcriptomic analysis of tissues and embryos from control and triglyceride lipase 2 (TL2)-deficient Anopheles gambiae females.

ABSTRACT: Transcriptomic analysis of tissues and embryos from control and triglyceride lipase 2 (TL2)-deficient Anopheles gambiae females.

PROVIDER: PRJNA1098770 | ENA |

REPOSITORIES: ENA

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Transcriptomic analysis of tissues and embryos from control and triglyceride lipase 2 (TL2)-deficient Anopheles gambiae females.

Project description:We explored the role of triglyceride (TAG) mobilization in the reproductive success of female Anopheles gambiae after a blood meal. Silencing triglyceride lipase 2 (TL2) in An. gambiae reduced egg production and abrogates fertility. Embryos from AgTL2-depleted females develop normally but fail to hatch and are characterized by reduced levels of lipids and key metabolites, including carbohydrates, Tricarboxylic acid (TCA) intermediates, paralleled by increased levels of purine and pyrimidine breakdown products, signifying possible cellular death. To obtain a better understanding of the mechanism underlying embryonic lethality in AgTL2-deficient females, we generated RNA-Seq datasets of tissues and embryos from control and TL2-knockdown females at different time points after blood feeding (for tissues) and oviposition. These analyses revealed that while at earlier time points there is almost no difference, transcriptomic profiles at the later time point of 38 hours after laying were profoundly distinct between both groups. Specifically, pathways involved in neuropeptide signaling, G-protein coupled receptor signaling, and eclosion hormone synthesis were repressed in TL2-knockdown condition while hallmarks of cellular stress were significantly up-regulated. More importantly, pathways involved in de novo lipid synthesis and transport were significantly up-regulated, possibly to compensate for reduced levels of maternally inherited lipids.

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Expression data from Anopheles gambiae females blood fed twice within a gonotrophic cycle

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Project description:We custom-built a bioinformatics pipeline to search for 20E-modifying enzymes in the accessory glands of Anopheles gambiae males, searching for ecdysteroid kinases (EcK), ecdysone oxidases (EO), and ecdysteroid-phosphate phosphatases (EPP). To this end, we generated RNAseq datasets of different An. gambiae tissues dissected from virgin and mated females and males, and produced similar datasets for Anopheles albimanus, a South American species that does not synthetize and transfer ecdysteroids during mating. These analyses led to the identification of one candidate EPP and two potential EcKs (EcK1 and EcK2), which we demonstrated are involved in the activity of a male-specific oxidized ecdysteroid (3D20E). We further determined that 3D20E is specifically produced by the An. gambiae male accessory glands and is transferred to females during copulation, where it triggers a series of post-mating responses.

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Transcriptomic analysis of Anopheles gambiae females subjected to physiological stress.

Project description:Transcriptomic analysis of Anopheles gambiae females subjected to physiological stress.

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Anopheles gambiae

Project description:Anopheles gambiae

| PRJNA36581 | ENA

Transcription profiling of Anopheles gambiae female bodies from virgin mosquitoes and 2, 6 and 24 hours after mating

Project description:Changes in gene expression in whole Anopheles gambiae female bodies between virgin mosquitoes and females samples at 2h, 6h, and 24h after mating.

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