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An Optimised Faecal microRNA Sequencing Pipeline Reveals Fibrosis in Trichuris muris Infection

ABSTRACT: An Optimised Faecal microRNA Sequencing Pipeline Reveals Fibrosis in Trichuris muris Infection

PROVIDER: PRJNA1111849 | ENA |

REPOSITORIES: ENA

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An Optimised Faecal microRNA Sequencing Pipeline Reveals Fibrosis in Trichuris muris Infection

Project description:The intestine is a site of diverse functions including digestion, nutrient absorption, immune surveillance, and microbial symbiosis. As such, intestinal homeostasis is vital for overall wellbeing. Faecal microRNAs (miRNAs) offer valuable non-invasive insights into the transcriptional state of the intestine. However, typical faecal miRNA yields and profiles remain incompletely characterised. Here, we develop an optimised protocol for faecal miRNA detection, and describe a reproducible murine faecal miRNA profile across several studies by performing a meta-analysis. By examining faecal miRNA changes during chronic infection with the gastrointestinal helminth, Trichuris muris, we identify the altered expression of miRNAs associated with fibrosis, barrier integrity and wound healing. Fibrosis was confirmed in vivo, suggesting a role for these miRNAs in regulating wound healing during chronic infection where the production of classical wound healing Th2 cytokines are low. Further implementations of this technique can identify novel hypotheses and therapeutic strategies in diverse disease contexts.

2024-12-20 | GSE267555 | GEO

Trichuris muris transcriptomics

Project description:Trichuris muris is very closely related to the human parasite T. trichiura sharing cross reactive antigens. Moreover, it is a remarkably tractable model system for dissecting immune responses and host parasite relationships and is actively being investigated in a number of laboratories worldwide. T. muris is a naturally occurring nematode parasite of mice which resides in the caecum and colon and has a direct oral faecal life cycle. High-throughput sequencing of Trichuris muris transcriptome for de novo assembly of transcripts. The main objective of this project is to recognize genes expressed in given life stages. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2013-05-28 | E-ERAD-125 | biostudies-arrayexpress

Trichuris muris

Project description:Trichuris muris overview

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Extracellular vesicles Trichuris muris

Project description:We analysed the exosomes secreted by the nematode Trichuris muris. Two replicates of exosomes were analysed using a 5600+ mass spectrometer

2017-12-13 | PXD008387 | Pride

mRNA data from extracellular vesicles secreted by Trichuris muris

Project description:RNA-Seq analysis of the mRNA present in the extracellular vesicles secreted by the nematode Trichuris muris

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miRNA data from extracellular vesicles secreted by Trichuris muris

Project description:miRNA-Seq analysis of the miRNA present in the extracellular vesicles secreted by the nematode Trichuris muris

2017-12-13 | GSE107985 | GEO

Extracellular vesicles from Heligmosomoides bakeri and Trichuris muris contain distinct small RNAs

Project description:We report the small RNA content of extracellular vesicles (EVs) from adult parasite Trichuris muris and Heligmosomoides bakeri

2020-08-01 | GSE152591 | GEO

Effect of B cell-intrinsic deletion of MLL1 on gene expression of germinal centre B cells during day 13 Trichuris muris infection

Project description:We measured the gene expression differences in germinal centre B cells from day 13 Trichuris muris-infected mice in which MLL1 is conditionally deleted in mature B cells

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Trichuris secretome - proteome data analysis

Project description:We analysed the excretory/secretory (ES) products and the exosomes secreted by the nematode Trichuris muris. Two batches of ES and Optiprep fractions of exosomes were analysed using a 5600+ mass spectrometer

2017-05-23 | PXD006344 | Pride

Transcription profiling of intestine from mice infected with three isolates of Trichuris muris for 7 days to determine early changes in gene expression that may later determine the outcome of infection

Project description:Trichuris muris, the mouse whipworm is used as a laboratory model of the human parasite T. trichuira - a gastrointestinal nematode. Three laboratory isolates of T. muris exist: the E, J and S isolates. Previous data has shown that the S isolate survives to chronicity in C57BL/6 mice unlike the E and J isolates which are expelled. The ability of the S isolate to persist is still unclear. Microarray analysis of gut samples from mice infected with either the E or S isolates of T. muris for 7 days was used to determine early changes in gene expression that may later determine the outcome of infection.

2010-02-26 | E-MEXP-1795 | biostudies-arrayexpress

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