Project description:Method development for protein extraction from microscopic biominerals. The method was developed using Hong Kong oyster larval shells
Project description:Here we reported 226 sperm proteins from the Hong Kong oyster Crassostrea hongkongensis. Proteins extracted from three sperm samples were separated by SDS-PAGE, analyzed by LC-MS/MS and identified using Mascot.
Project description:We have worked on skin explants and activated T cells locally with a CD3 antibody, whole biopsies were activated, then epidermal and dermal RNA was sequenced. Sequencing was performed by BGI (Hong Kong) as well as the group analysis.
Project description:We report changes in H3K27ac following LPS stimulation in Detroit 562 cells. We were able to identified LPS-increased H3K27ac regions which correlated with RELA binding as well as gene up-regulation. This data set is relevant for airborne bacterial sensing as Detroit 562 cells are nasopharyngeal epithelial cells and LPS is a gram negative bacterial endotoxin.
Project description:Guanidine DNA quadruplex (G4-DNA) structures convey a distinctive layer of epigenetic information that is critical for the regulation of key biological activities and processes as genome transcription regulation, replication and repair. Despite several works that have been published recently, the information regarding their role and possible use as therapeutic drug targets in bacteria is still scarce. Here, we tested the biological activity of a small G4-DNA ligand library based on the naphthalene diimide (NDI) pharmacophore, against both Gram-positive and Gram-negative bacteria. For the best compound identified, NDI-10, the action mechanism was further characterized. Gram-negative bacteria were more resistant altogether due to the presence of the outer membrane, although the activity of the G4-Ligand was generally bactericidal, while it was bacteriostatic for Gram-positive bacteria. This asymmetric activity could be related to the different prevalence of putative G4-DNA structures in each group, the influence that they can exert on the gene expression (which was found more severe for the Gram-negative bacteria) and the role of the G4 structures in these bacteria, that seems to be more related to promote transcription in Gram-positive bacteria and repress transcription in Gram-negative.
Project description:RNA sequencing was carried out at BGI, Hong Kong on an Illumina HiSeq platform to compare gene expression in Acinetobacter baumannii strain S1 and an adeAB deletion mutant in this strain.
Project description:This animal study was approved by the Ethics Committee at school of Chinese medicine,Hong Kong Baptist University. A total of 4 female and 4 male C57BL/6 mices were included in control diet group (BC); A total of 4 female and 4 male C57BL/6 mices were included in high fat diet group (BT).
