Project description:To get insight of molecular mechanism of GPR39, we investigated transcriptomic response in GPR39 overexpressed HEK293 cells after TC-G-1008 stimulation We identified 814 differentially expressed genes (DEGs) fold change cutoff = 1.5 in the GPR39 overexpressed HEK293 and GPR39 overexpressed HEK293 cells with TC-G-1008 treatment, those including 364 up-regulated and 450 down-regulated genes These findings suggest that MAPK/Erk, PI3K/Akt and Glycerolipid metabolism signaling pathways would be putative signaling pathways dominantly altered by GPR39 activation.
Project description:In order to gain insight into the molecular mechanism of PROKR1, we investigated transcriptomic response in PROKR1 overexpressed HEK293 T cells following Pk2 stimulation We identified 578 differentially expressed genes (DEGs) fold change cutoff = 2 in activated HEK293 T overexpressed HEK293 T cells by Pk2, including 269 up-regulated and 309 down-regulated genes.
Project description:Wild-type and RBMX-overexpressed HEK293 cells where profiled at the transcriptome and translatome levels through polysomal profiling. Paired-end RNA-seq libraries were built and sequenced on an Illumina HiSeq 2000 machine to allow for accurate gene expression quantification and alternative splicing isoforms detection.
Project description:Human lactoferrin (LF) is a multifunctional protein involved in immunomodulation, cell growth, and differentiation. In addition to the secreted form (sLF), an alternatively spliced form (ΔLF) that lacks the signal sequence and downregulated in cancer was found. This study was carried out to identify and compare signaling networks provoked by the two LF isoforms. To do this, the two forms were overexpressed in HEK293 cells using the flp-in/tet-on system and genome-wide expression analysis of 18,367 genes was conducted. Secreted form (sLF) or alternatively spliced form (ΔLF) were overexpressed in HEK293 cells using the flp-in/tet-on system and genome-wide expression analysis of 18,367 genes was conducted.
Project description:Flag-tagged rat GluA3 and rat alpha2delta-1 (DNA ratio: 5:1) was transiently expressed in HEK293 cells. MG-132 was applied overnight before sample harvesting. After 48 hours of transfection, the cells were collected, and flag tagged GluA3 along with its interacting proteins were enriched through immunoprecipitation using a rabbit anti flag antibody, and then subjected to mass spectrometric analyses.
Project description:To gain insight into the function of Nuclear pore associated protein 1 (NPAP1, formerly C15orf2), we overexpressed NPAP1 in HEK293 cells. We detected no significant difference between NPAP1-expression of induced and uninduced cells in three technical replicates, exept for an approximately 10-fold increase in the NPAP1 transcript itself. This indicates that overexpression of NPAP1 does not change mRNA expression profiles of HEK293 cells. We used microarrays to investigate global gene expression changes depending on the level of NPAP1/C15orf2
Project description:To gain insight into the function of Nuclear pore associated protein 1 (NPAP1, formerly C15orf2), we overexpressed NPAP1 in HEK293 cells. We detected no significant difference between NPAP1-expression of induced and uninduced cells in three technical replicates, exept for an approximately 10-fold increase in the NPAP1 transcript itself. This indicates that overexpression of NPAP1 does not change mRNA expression profiles of HEK293 cells. We used microarrays to investigate global gene expression changes depending on the level of NPAP1/C15orf2 We compared NPAP1 overexpressing cells to untreated cells, which do not express detectable amounts of NPAP1 protein, to determine global gene expression changes.