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Homo sapiens

ABSTRACT: Expression data from NS398-treated and control HT29 colon adenocarcinoma cell line samples

OTHER RELATED OMICS DATASETS IN: E-GEOD-15799

PROVIDER: PRJNA117015 | ENA |

REPOSITORIES: ENA

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E-GEOD-15799

Project description:Expression data from NS398-treated and control HT29 colon adenocarcinoma cell line samples

2014-07-03 | E-GEOD-15799 | ExpressionAtlas

Expression data from NS398-treated and control HT29 colon adenocarcinoma cell line samples

Project description:The whole-genome oligonucleotide microarray analysis of NS398-treated HT29 colon adenocarcinoma cells samples can give an insight into global molecular background of selective COX2 inhibitor administration in order to find other target molecules and pathways influenced by NS398 selective COX2 inhibitor treatment in the epithelial cells. Total RNA was extracted from NS398-treated and control HT29 colon adenocarcinoma cells and hybridized on Affymetrix HGU133 Plus 2.0 microarrays

2010-03-16 | E-GEOD-15799 | biostudies-arrayexpress

Real-time quantitative PCR analysis of human colon cancer cells for UPR related genes

Project description:human colon cancer cell line HT29 cells treated with 0.5μM thapsigargin 2.5hours. Compared with equal amount total RNA from nontreated control cells. qPCR gene expression profiling.

2013-09-25 | GSE51140 | GEO

Real-time quantitative PCR analysis of human colon cancer cells for autophagy related genes

2013-09-25 | GSE51141 | GEO

Expression data from NS398-treated and control HT29 colon adenocarcinoma cell line samples

2010-03-03 | GSE15799 | GEO

Real-time quantitative PCR analysis of human colon cancer cells for UPR related genes

Project description:human colon cancer cell line HT29 cells treated with 0.5?M thapsigargin 2.5hours. Compared with equal amount total RNA from nontreated control cells. qPCR gene expression profiling. one sample

2013-09-25 | E-GEOD-51140 | biostudies-arrayexpress

Real-time quantitative PCR analysis of human colon cancer cells for autophagy related genes

2013-09-25 | E-GEOD-51141 | biostudies-arrayexpress

Transcriptome analysis of novel CPT-CEF-treated HT29 colon cancer cells to evaluate drug cytotoxicity

Project description:Purpose: To identify differentially expressed genes in HT29 colon cancer cells after treatment with a novel formulation of camptothecin with β-cyclodextrin-EDTA-Fe3O4 nanoparticle-conjugated nanocarriers (CPT-CEF) Methods:Treated HT29 cell lines with CPT-CEF, isolated total RNA from HT29 colon cancer cells, and prepared library for RNA sequencing. Carried out comparative transcriptomic studies between treated and untreated cells to find out which gene functions were dysregulated by CPT-CEF. Results: The study yielded 247 DEGs ((FDR<0.05, FC>2.0) that were affected by CPT-CEF treatment in the HT29 colon cancer cells. The results obtained from cell cycle analysis, mitochondrial depolarization assay and acridium orange/propidium iodide double staining showed potential of CPT-CEF in cancer cell inhibition. Conclusion: Our study successfully identified DEGs in the CPT-CEF treated HT29 colon cancer cells that pointed to inhibition of cancer progression. To further affirm, animal studies are needed.

2021-09-15 | GSE165875 | GEO

Homo sapiens

Project description:Expression data from HT29 intestinal epithelial cell line

| PRJNA242398 | ENA

Transcriptome analysis in HT29 and SW480 cells depleted of Prdx2

Project description:Prdx2 is the thioredoxin-dependent peroxidase that reduces H2O2 using reducing power NADPH in the presence of thioredoxin and thioredoxin reductase. Prdx2 plays an important role in growth. factor signaling in mammlian cells. Therefore, we examined the gene expression in colon adenocarcinoma cell line HT29 after Prdx2 depletion. Prdx2 depletion resulted in a significant alteration on gene expression, including protein synthesis, metabolisms, and cell cycle.

2017-05-13 | GSE81429 | GEO

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