Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Mycobacterium tuberculosis ESX phase variant barcoded minipool mouse infection

PROVIDER: PRJNA1182320 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR31231469.fastq.gz	Fastqsanger.gz
	SRR31231470.fastq.gz	Fastqsanger.gz
	SRR31231471.fastq.gz	Fastqsanger.gz
	SRR31231472.fastq.gz	Fastqsanger.gz
	SRR31231473.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Gene expression profile of M.tuberculosis espR (Rv3849) mutant

Project description:Mycobacterium tuberculosis, a pathogen of global importance, utilizes the ESX-1 protein secretion system to export virulence factors that disarm host macrophages. Although this secretory pathway is critical for virulence, how ESX-1 is regulated is completely unknown. Here we show that EspR (Rv3849) is a key regulator of ESX-1. EspR activates transcription of an operon that includes three ESX-1 components, Rv3616c-Rv3614c, whose expression in turn promotes secretion of ESX-1 substrates. Keywords: Strain comparison Comparison of the global gene expression of three M.tb strains grown in log phase: Erdman strain of M.tb, espR transposon mutant, and the espR transposon mutant complemented with the espR gene

2008-07-04 | E-GEOD-11696 | biostudies-arrayexpress

Comparative analysis of the gene expression profiles of M.tuberculosis espR (Rv3849) transposon and knockout mutants

2010-05-25 | E-GEOD-12381 | biostudies-arrayexpress

RNAseq data for murine BMDM infected with wild-type Mycobacterium tuberculosis, a PDIM mutant, or an ESX-1 mutant.

Project description:C57BL6 murine bone marrow-derived marcrophages were infected with Mycobacterium tuberculosis H37Rv, a PDIM mutant (H37RvΔmas) or an ESX-1 mutant (Tn::eccCa1) strains at an MOI of 2:1. RNA was harvested at 4, 8, 12, and 16 hours post-infection for comprehensive transcriptional profiling.

2021-11-10 | GSE144330 | GEO

Gene expression profile of M.tuberculosis espR (Rv3849) mutant complemented with an N-terminal Flag espR gene

Project description:Mycobacterium tuberculosis, a pathogen of global importance, utilizes the ESX-1 protein secretion system to export virulence factors that disarm host macrophages. Although this secretory pathway is critical for virulence, how ESX-1 is regulated is completely unknown. Here we show that EspR (Rv3849) is a key regulator of ESX-1. EspR activates transcription of an operon that includes three ESX-1 components, Rv3616c-Rv3614c, whose expression in turn promotes secretion of ESX-1 substrates. Keywords: Strain comparison Comparison of the global gene expression of four M.tb strains grown in log phase: Erdman strain of M.tb, espR transposon mutant, the espR transposon mutant complemented with the wild type copy of the espR gene, and the espR transposon mutant complemented with an N-terminal FLAG espR gene.

2008-08-08 | E-GEOD-12379 | biostudies-arrayexpress

Gene expression profile of M.tuberculosis espR (Rv3849) mutant complemented with an N-terminal Flag espR gene

2008-08-08 | GSE12379 | GEO

Comparative analysis of the gene expression profiles of M.tuberculosis espR (Rv3849) transposon and knockout mutants

2008-08-08 | GSE12381 | GEO

Gene expression profile of M.tuberculosis espR (Rv3849) mutants containing N- or C-terminal mutations in the espR gene

2008-08-08 | GSE12380 | GEO

Gene expression profile of M.tuberculosis espR (Rv3849) mutant

2008-07-04 | GSE11696 | GEO

Transcriptional profile of Mycobacterium tuberculosis espL deletion mutant

Project description:The ESX-1, type VII, secretion system represents the major virulence determinant of Mycobacterium tuberculosis. The ESX-1 cluster comprises approximately twenty genes and encodes a specialized secretion apparatus, which releases effectors into the extracellular milieu. The goal of this study is to understand the role of EspL. We show that EspL, a protein of 115 amino acids, is essential for mediating ESX-1-dependent virulence and for stabilization of EspE, EspF and EspH protein levels. Indeed, an espL knock-out mutant was unable to replicate intracellularly, secrete ESX-1 substrates or stimulate innate cytokine production. Moreover, loss of EspL also leads to downregulation in M. tuberculosis of WhiB6, a redox-sensitive transcriptional activator of ESX-1 genes.

2018-12-07 | GSE118994 | GEO

Transcriptional profile of Mycobacterium tuberculosis mycP1-eccE1 deletion mutant

Project description:Mycobacterium tuberculosis is a slow-growing intracellular bacterium with the ability to induce host cell death and persist indefinitely in the human body. This pathogen uses the specialised ESX-1 secretion system to secrete virulence factors and potent immunogenic effectors required for disease progression. ESX-1 is a multi-subunit apparatus with a membrane complex that is predicted to form a pore in the cytoplasmic membrane. In M. tuberculosis this complex is made of five membrane proteins: EccB1, EccCa1, EccCb1, EccD1, EccE1. In this study, we have characterised the membrane component EccE1, and we found that deletion of eccE1 leads to disruption of ESX-1 secretion and attenuation of M. tuberculosis ex vivo.

2019-09-01 | GSE119582 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data