Project description:Total RNA was isolated from all the placental tissues using Trizol, and mRNA was isolated using FastTrack Kit. We used a placenta reference as a standard for all these array hybridizations. The placenta reference is a mixture of placenta mRNA with CRG.
Project description:Total RNA was isolated from all the placental tissues using Trizol, and mRNA was isolated using FastTrack Kit. We used a placenta reference as a standard for all these array hybridizations. The placenta reference is a mixture of placenta mRNA with CRG. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Computed
Project description:The placenta regulates maternal-fetal communication, and its defect leads to significant pregnancy complications. The maternal and embryonic circulations are primitively connected in early placentation, but the function of the placenta during this developmentally essential period is relatively unknown. We thus performed a comparative proteomic analysis of the placenta before and after primary placentation and found that the metabolism and transport of lipids were characteristically activated in this period. The placental fatty acid (FA) carriers in specific placental compartments were upregulated according to gestational age, and metabolomic analysis also showed that the placental transport of FAs increased in a time-dependent manner. Further analysis of two mutant mice models with embryonic lethality revealed that lipid-related signatures could reflect the functional state of the placenta. Our findings highlight the importance of the nutrient transport function of the primary placenta in the early gestational period and the role of lipids in embryonic development.
Project description:Total RNA was isolated from all the placental tissues using Trizol, and mRNA was isolated using FastTrack Kit. We used a placenta reference as a standard for all these array hybridizations. The placenta reference is a mixture of placenta mRNA with CRG. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set
Project description:To find potential biomarkers and molecular mechanism for placenta accreta spectrum disorders , we identified the differently expressed patterns of lncRNAs and mRNAs in invasive placneta and adherent normal placenta tissues. The results provided a novel insight into the pathogenesis of placenta accreta spectrum disorders .
Project description:In this study, the molecular signature of placenta membrane from preterm birth placenta was assessed and compared to full-term placenta by proteomic profiling with the aim to identify molecules relevant to preterm birth.