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Drosophila melanogaster

ABSTRACT: Expression profiling of over-expression of conspecific and heterspecific miR-310 cluster in Drosophila (tiling array)

OTHER RELATED OMICS DATASETS IN: GSE15863

PROVIDER: PRJNA123053 | ENA |

REPOSITORIES: ENA

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Drosophila melanogaster

Project description:Expression profiling of over-expression of conspecific and heterspecific miR-310 cluster in Drosophila (cDNA array)

| PRJNA123049 | ENA

Expression profiling of over-expression of conspecific and heterspecific miR-310 cluster in Drosophila (tiling array)

Project description:The miRNA 310-311-312-313 cluster from D. melanogaster (referred to as Dm310s) and the homolog from D. pseudoobscura (Dp310s) were used to transform D. melanogaster. The over-expression of UAS-miR310s in two Dm310s transgenic lines (M1-7 and M1-3) and Dp310s transgenic line P4-18 was driven by GAL4 line NP5941 with native miR-310 expression pattern. We used Drosophila Tiling 1.0 F arrays to assess the effects of miR310s over-expression on the whole transcriptome. We compared the transcriptional profiling of Dm310s and Dp310s overexpression in D.melanogaster using double-stranded cDNA followed by bioprime random labeling, and hybridization to Affy Drosophila tiling 1.0 F array. Third-instar progeny larvae from the crosses with maternal NP5941 and paternal UAS-miR-310 cluster transgenic lines were collected and third-instar larvae from the cross with maternal NP5941 and paternal w1118 were used as control. Three biological repeats were used for each stock. A processed data matrix reporting values (log2 intensity after quantile normalization) for all of the Samples is linked below as a supplementary file.

2010-06-24 | E-GEOD-15850 | biostudies-arrayexpress

Expression profiling of over-expression of conspecific and heterspecific miR-310 cluster in Drosophila (tiling array)

2010-06-25 | GSE15850 | GEO

Expression profiling of dme-miR-310 cluster (miR-310, miR-311, miR-312, miR-313) knockout

Project description:Expression profiling of the dme-miR-310 cluster knockout line in D.melanogaster. The knockout line was generated in an imprecise P-element excision screen using a stock P{EP}2587 containing a P-element insertion directly upstream of the miR-310 cluster. The precise excision line of P{EP}2587 was used as the control. The expression of miR-310 cluster knockout line (imprecise deletion of P{EP}2587 insertion) were compared with the control (precise deletion of P{EP}2587 insertion) using Drosophila Tiling 1.0 F array. Each stock has three biological repeats. A processed data matrix reporting values (log2 intensity after quantile normalization) for all of the Samples is linked below as a supplementary file.

2010-06-24 | E-GEOD-15851 | biostudies-arrayexpress

Drosophila melanogaster

Project description:Expression profiling of dme-miR-310 cluster (miR-310, miR-311, miR-312, miR-313) knockout

| PRJNA123055 | ENA

Drosophila melanogaster miR310 cluster over-expression and knockout

Project description:We tested and measured the strength of heterospecific interactions between microRNAs and their targets by carrying out transgenic experiments across Drosophila species, by over-expressing the miR310s cluster of D. melanogaster (Dm310s) and D. pseudoobscura (Dp310s) in D. melanogaster. A knockout line, referred to as Dm310s (−), was also included for comparison. The whole-genome expression (WGE) of the Dp310s and Dm310s transgenic lines were compared on two platforms of both Affymetrix tiling array and DGRC cDNA array. The WGE of knockout line was assayed on only Affymetrix tiling array. In the Dm310s lines, overexpression was more common than underexpression among misregulated genes while the pattern appeared to be reversed in the Dm310s(−) line. The number of genes significantly misexpressed under the influence of Dp310s is 3-10 times greater than under Dm310s. Importantly, the numbers of predicted targets are similar between them. Expression analysis of the predicted target genes suggests that miRNAs may sometimes function to buffer fluctuations in the transcriptome output. After the buffering function has evolved, heterospecific combinations may cause adverse effects. This SuperSeries is composed of the SubSeries listed below.

2010-06-25 | GSE15863 | GEO

Expression profiling of over-expression of conspecific and heterspecific miR-310 cluster in Drosophila (cDNA array)

2010-06-25 | GSE15847 | GEO

Expression profiling of dme-miR-310 cluster (miR-310, miR-311, miR-312, miR-313) knockout

2010-06-25 | GSE15851 | GEO

Expression profiling of over-expression of conspecific and heterspecific miR-310 cluster in Drosophila (cDNA array)

2010-06-25 | E-GEOD-15847 | biostudies-arrayexpress

Drosophila melanogaster

Project description:Tiling Array HSF immunopurification

| PRJNA91141 | ENA