Project description:We built 3 strains from Candida albicans wild-type strains, mutant strain with one TRK1 gene deleted(one allele of two genes was deleted), overexpression strain(add one allele of the gene to rp10 locus), and restoration strain(add one allele of the gene to mutant strains). The goal is study the expression level change of genes among the three strains compared with wild-type strain on a genomic scale. Keywords: genetic modification
Project description:The experiment was designed to study the transcriptional profiling of Candida albicans wild-type CAI-4 strains and corresponding Histatin 5 treated strains. Since Histatin 5 will kill Candida albicans, the focus will be on how many and which genes are significantly affected with the treatment of Histatin 5. Currently, we only conducted the microarray experiments at one concentration of Histatin 5 and one time point of treatment. Keywords: cell type comparison
Project description:Transcriptional profiling of Candida albicans cells comparing control untreated C. albicans cells with sulfite-treated C. albicans cells. Sulfite is a toxic molecule that C. albicans encounters in its human host. Both wild type and ∆zcf2 mutant cells were used. The goal was to determine the effects of sulfite on C. albicans gene expression, and to determine which of the genes areZcf2-depedent.
Project description:Transcriptomic analysis of wild type and GCN5-deficient Candida albicans cells prior to and after treatment with Caspofungin 15 min and 45 min was done to determine the transcriptional changes in these strains
Project description:We perform microarray analysis of HUVECs upon stimulation with virulent wildtype C. albicans strain SC5314 or its efg1/efg1 cph1/cph1 hyphal-deficient derivative strain CAN34 to compare the gene expression profiles elicited from HUVECs in response to these strains. In addition, these responses are compared to that of TNF-alpha induced responses to determine which responses are Candida-specific. Keywords: comparison of host response to different Candida albicans morphologies
