Project description:Pien Tze Huang (PZH) is herbal traditional Chinese medicine which was widely utilized in Asia for hepatic diseases. We constructed two groups hepatic fibrosis mice model using CCl4, one group using PZH treatment and another group replacing PZH with double distilled water. All 12 mice were fed for 8 weeks and then killed to have their liver tissue taken out. Small RNA-seq were used to identify miRNAs of PZH medicine effect for hepatic fibrosis. We found the expression of these miRNAs (mmu-miR-205-5p, mmu-miR-3064-5p, mmu-miR-205-5p, mmu-miR-370-3p, mmu-miR-665-3p) were changed in PZH medicine treatment for hepatic fibrosis study. Furthermore, Hmga2 and Fgf9, miRNAs corresponding target genes (Sp4, Slc2a6, Tln2, Hmga2, Ank3, Pax9, Fgf9), have been reported association with hepatic fibrosis.
Project description:To identify biomarkers regulated by traditional Chinese medicine Astragalus membranaceus Fischer Bge. var. mongolicus Bge. Hsiao in colorectal cancer. We have identified several differentially expressed genes including microRNAs using Affymetrix HTA-2.0 array. In this dataset, we include the expression data obtained from colon cancer cell line HCT116 grafted into nude mice. The mice was treated either water or traditional Chinese medicine Astragalus membranaceus for 28 days. These data are used to obtain 1425 genes that are differentially expressed in response to Astragalus membranaceus treatment.
Project description:Potentilla indica and Melastoma dodecandrum lour are medicinal herbs used in traditional Chinese medicine. We sampled the plants from Nanyang Technological University's herb garden for transcriptomics analysis.
Project description:Capsicum frutescens is one of the chilli varieties, with erect, highly pungent fruits. This variety is used to prepare hot sauces, chilli concentrates, and oleoresin. Its fruits are also used in traditional Indian medicine to treat arthritis and zoster related pain management. Infection by Polyphagotarsonemus latus commonly called as the Broad mite in chilli, make the leaves turn coppery and dark, show symptoms of leaf curl and stunted growth that eventually results in low yield and economic loss. We carried out gel-free, labelled mass spectrometry-based quantitative proteomic analysis of leaves and apical meristems of healthy and infected plants. We identified a total of 5799 proteins, of which total of which, 1677 proteins found to dysregulated in infested plants.
Project description:The myocardial tissues of the ischemic left ventricle and the peri-ischemic rim (an approximately 1 mm rim of normal-appearing tissues) were removed, and the remaining tissues that consisted of non-ischemic left ventricle were obtained for the transcriptional analysis. The total RNA was extracted from the myocardia from the sham (n = 3), myocardial ischemia model (n = 3), and a traditional Chinese medicine formula treated on myocardial ischemia (n = 3) groups. Beads that contained oligo were used to isolate the poly mRNA from the total RNA and then the rRNA was subsequently removed. The amplified library was sequenced on a HiSeq 2500 sequencing machine (Illumina, San Diego, California, USA) with a 50 bp SE strategy. Using CASAVA software, the images generated by the Illumina sequencers were converted into raw reads by base-calling.