Project description:Transcriptional profiling of Arabidopsis thaliana seedlings treated with cis-cinnamic acid derivatives constructed by Nishikawa et al. (2013) Phytochemistry (http://dx.doi.org/10.1016/j.phytochem.2013.08.013), evaluating the candidate of herbicide chemicals by observing early response of gene expressions in Arabidopsis seedlings.
Project description:To understand the role of GCN2 in regulating translation, we compared the polysome loading state and overall transcript level between Arabidopsis thaliana wild type (ecotype Landsberg erecta) and gcn2 (Genetrap line GT8359, Cold Spring Harbor Laboratory) seedlings with or without herbicide chlorosufuron treatment RNA was fractionated using sucrose gradients into polysomal and nonpolysomal RNAs. We also determined overall total transcript levels. We used Affymetrix ATH1 microarrays.
Project description:Transcriptional profiling of Arabidopsis wild-type (Col0) control seedlings with corresponding mutant seedlings is performed using Aligent's Whole Arabidopsis Gene Expression Microarray (G2519F, V4, 4x44K).
Project description:Dicamba is an auxin-like herbicide that can stimulate the production of ethylene and ABA biosynthesis. The subsequent stomatal closure and build-up of reactive oxygen species is hypothesized to contribute to plant death. In order to further understand the herbicide dicamba's mode of action at the molecular level, we used microarrays to dissect global gene expression in Arabidopsis seedlings 10 hrs after dicamba treatment. 4 day old Arabidopsis seedlings (a Columbia line containing a -800GSTF8::LUC reporter) were flooded with water or 7mM dicamba for 40 minutes before the treatment was poured off. Ten hours after the start of the treatment, whole seedlings were collected
Project description:Transcriptional profiling of Arabidopsis wild-type (Col0) control flower buds or seedlings with corresponding mutant flower buds or seedlings is performed using Aligent's Whole Arabidopsis Gene Expression Microarray (G2519F, V4, 4x44K).
Project description:Transcriptional profiling of 6-day-old seedlings of Arabidopsis wild type control and zrf1 mutants is performed using Agilent's Whole Arabidopsis Gene Expression Microarray (4x44K).
Project description:Transcriptional profiling of Arabidopsis thaliana seedlings treated with cis-cinnamic acid derivatives constructed by Nishikawa et al. (2013) Phytochemistry (http://dx.doi.org/10.1016/j.phytochem.2013.08.013), evaluating the candidate of herbicide chemicals by observing early response of gene expressions in Arabidopsis seedlings. Two-condition experiment, cis-CA analogue-treated seedlings vs. control seedlings. Biological replicates:2 control replicates, 2.