Project description:We compared the expression between inter-specific hybrids (A. thaliana-A. lyrata, A. thaliana-A. halleri) and mid parent values of their parental lines. Mature leaves in A. thaliana (Col), A. lyrata ssp. lyrata, A. halleri ssp. gemmifera and interspecific hybrid between A. thaliana and A. lyrata and between A. thaliana and A. halleri
Project description:The mature leaves (full-expanded leaves) and developing leaves (30%length of full-expanded) of 8 species of the genus Flaveria (F.pringlei, F.robusta, F.floridana, F.ramosissima, F.brownii, F.palmeri, F.bidentis and F.trinervia) Transcriptome analysis. Plants were grown in the growth chamber (12hr light - 12hr dark, 24℃, 200-300umol m-2 s-1). Total RNA was isolated from the youngest full expanded leaves or developing leaves (30%length of full-expanded) of each species.
Project description:We compared the expression between inter-specific hybrids (A. thaliana-A. lyrata, A. thaliana-A. halleri) and mid parent values of their parental lines.
Project description:Assembly of the plant OXPHOS complexes was studied using complexome profiling. mitochondria were extracted from seedlings or mature leaves of the reference organism Arabidopsis thaliana. In seedlings, a fast-growing tissue, mitochondria biogenesis is very active whereas in mature leaves, little biogenesis is occurring. Comparison of both datasets allow the identification of assembly intermediates that are accumulating in seedlings but not in leaves.
Project description:Transcriptional profiling of Arabidopsis thaliana, comparing control wild-type (ecotype Wassilewskija, Ws) leaves with leaves from transgenic plants overexpressing the transcription factor RAP2.6L under control of the cauliflower mosaic virus 35S promoter (RAP2.6L-OX; this line was originally described in Krishnaswamy et al (2010)).
Project description:Overexpression of KlaxMUTE1 induced asymmetric, subsidiary-cell-like divisions. To determine the downstream target genes induced, an RNA-sequencing experiment of mature wild-type and mature leaves that overexpress KlaxMUTE1 and show many ectopic asymmetric cell divisions was performed.
Project description:We systematically identified long noncoding natural antisense transcripts (lncNATs), defined as lncRNAs transcribed from the opposite DNA strand of coding or noncoding genes. We identified in total 37,238 sense-antisense transcript pairs and found 70% mRNAs are associated with antisense transcripts in Arabidopsis. To detect the expression levels of these NAT pairs, we designed an Agilent custom array, ATH NAT array, and analyzed RNA samples from Arabidopsis inflorescences, leaves and roots, with 3 biological replicates each.
Project description:In order to better understand the transcriptional networks triggered by pathogen inoculation, we monitored gene expression in leaves of mutant Arabidopsis plants, inoculated with Pseudomonas syringae ES4326 and wild type Col-0 plants grown in parallel. Individual leaves were injected in the morning using a needle-less syringe with 10E5 cfu cm-2 PsmES4326 (suspended in 5 mM MgSO4). For the wild type, leaves were also mock treated with 5 mM MgSO4. Leaves were harvested 24 hours later. Plants were grown in pots with BM-2 soil (Berger Peat Moss Ltd, Quebec, Canada) at a density of 9 plants per pot and kept at 22 degrees Celsius with 75% humidity and a 12 hour day length. Keywords: Expression profilling by array