Project description:Transcriptional profiling of Arabidopsis thaliana seedlings treated with auxin (indole-3-acetic acid), highlighting to the physiological function of auxin by observing early response of gene expressions in Arabidopsis seedlings. Two-condition experiment, auxin-treated seedlings vs. control seedlings. Biological replicates:2 control replicates, 2 auxin-treated.
Project description:Comparing Arabidopsis plants gene expresion in normal conditions (control) with acetic acid treated plants (acetic). Plants were grown on liquid MS media for 13 days, then they were transfered to MS liquid media (control) or MS+3,5 acetic acid (acetic) for two hours. Two-condition experiment, Control vs Acetic. Biological replicates: 4 control, 4 treated with acetic, independently grown. One replicate per array.
Project description:Comparing Arabidopsis plants gene expresion in normal conditions (control) with acetic acid treated plants (acetic). Plants were grown on liquid MS media for 13 days, then they were transfered to MS liquid media (control) or MS+3,5 acetic acid (acetic) for two hours.
Project description:Transcriptional profiling of Arabidopsis thaliana seedlings treated with auxin (indole-3-acetic acid), highlighting to the physiological function of auxin by observing early response of gene expressions in Arabidopsis seedlings.
Project description:Purpose: To compare transcriptomic changes after guvermectin (GV), indole-3-acetic acid (IAA), trans-zeatin (ZT), epibrassinolide (eBL) and gibberellin acid 3 (GA3) treatments in Arabidopsis. Method: 7-day-old MS liquid cultivated Col-0 seedlings were treated by 10 μM GV, 1 μM IAA, 1 μM ZT, 1 μM eBL or 1 μM GA3. Chemicals were added into MS liquid respectively and whole seedling samples were collected after treatment for 5, 30, 180 minutes. In total, we got control group and 5 treatment groups. Three biological replicates were performed. Results: In total, 54 samples were used for RNA sequencing analysis. At least 6 G clean bases were generated for each sample. Comparative analysis identified differences between GV and other plant growth regulators in transcriptional level.
