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MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing

ABSTRACT: MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing

PROVIDER: PRJNA147395 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR363956.fastq.gz	Fastqsanger.gz
	SRR363957.fastq.gz	Fastqsanger.gz
	SRR363958.fastq.gz	Fastqsanger.gz
	SRR363959.fastq.gz	Fastqsanger.gz
	SRR363960.fastq.gz	Fastqsanger.gz

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MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing

Project description:This SuperSeries is composed of the following subset Series: GSE32180: MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [microarray] GSE32184: MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [deep sequencing] Refer to individual Series

2011-11-30 | E-GEOD-32183 | biostudies-arrayexpress

MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [microarray]

Project description:Here we show that MIWI is a small RNA-guided ribonuclease (Slicer) that requires extensive complementarity for target cleavage in vitro. Disruption of its catalytic activity in mice by a single point mutation results in male infertility and displays increased accumulation of LINE1 transposon transcripts. Round spermatids were purified from adult mouse testes of indicated Miwi genotypes. Total RNA was extracted and subjected to microarray analysis for gene expression profiling.

2011-11-30 | E-GEOD-32180 | biostudies-arrayexpress

MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [microarray]

2011-11-30 | GSE32180 | GEO

MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [deep sequencing]

Project description:Here we show that MIWI is a small RNA-guided ribonuclease (Slicer) that requires extensive complementarity for target cleavage in vitro. Disruption of its catalytic activity in mice by a single point mutation results in male infertility and displays increased accumulation of LINE1 transposon transcripts. MIWI-associated piRNAs from different genotypes were sequenced. Total RNA from purified round spermatids were subjected to Ribozero purification and strand-specific RNAseq lib prepared. Global 5' RACE library was prepare from indicated genotypes.

2011-11-30 | E-GEOD-32184 | biostudies-arrayexpress

MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [deep sequencing]

2011-11-30 | GSE32184 | GEO

Mus musculus

Project description:MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [microarray]

| PRJNA154867 | ENA

A role for Fkbp6 and the chaperone machinery in piRNA amplification and transposon silencing

Project description:Epigenetic silencing of transposons by Piwi-interacting RNAs (piRNAs) constitutes an RNA-based genome defense mechanism. Piwi endonuclease action amplifies the piRNA pool by generating new piRNAs from target transcripts by a poorly understood mechanism. Here, we identified mouse Fkbp6 as a factor in this biogenesis pathway delivering piRNAs to the Piwi protein Miwi2. Mice lacking Fkbp6 derepress LINE1 (L1) retrotransposon and display reduced DNA methylation due to deficient nuclear accumulation of Miwi2. Like other co-chaperones, Fkbp6 associates with the molecular chaperone Hsp90 via its tetratricopeptide repeat (TPR) domain. Inhibition of the ATP-dependent Hsp90 activity in an insect cell culture model results in the accumulation of short antisense RNAs in Piwi complexes. We identify these to be by-products of piRNA amplification that accumulate only in nuage-localized Piwi proteins. We propose that the chaperone machinery normally ejects these inhibitory RNAs, allowing turnover of Piwi complexes for their continued participation in piRNA amplification.

2012-09-04 | GSE39203 | GEO

MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing

Project description:This SuperSeries is composed of the SubSeries listed below.

2011-11-30 | GSE32183 | GEO

Mus musculus

Project description:MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [deep sequencing]

| PRJNA154861 | ENA

Developmentally regulated piRNA clusters implicate MILI in transposon control

Project description:Nearly half of the mammalian genome is composed of repeated sequences. In Drosophila, PIWI proteins exert control over transposons. However, mammalian PIWI proteins, Miwi and Mili, partner with piRNAs that are depleted of repeat sequences, raising questions about a role for mammalian PIWIs in transposon control. A search for murine small RNAs that might program PIWI proteins for transposon suppression revealed developmentally regulated piRNA loci, some of which resemble transposon master control loci of Drosophila. We also find evidence of an adaptive amplification loop in which PIWI catalyzes formation of piRNA 5’ ends. Mili mutants de-repress L1 and IAP and lose DNA methylation of L1 elements, demonstrating an evolutionarily conserved role for PIWI proteins in transposon suppression. Keywords: small RNA profile, piRNA

2007-04-20 | GSE7414 | GEO

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