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Rattus norvegicus

ABSTRACT: Genome-wide analysis of EGR2/SOX10 binding in myelinating peripheral nerve

PROVIDER: PRJNA150811 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR399325.fastq.gz	Fastqsanger.gz
	SRR399326.fastq.gz	Fastqsanger.gz
	SRR399327.fastq.gz	Fastqsanger.gz
	SRR399328.fastq.gz	Fastqsanger.gz
	SRR399329.fastq.gz	Fastqsanger.gz

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Genome-wide analysis of EGR2/SOX10 binding in myelinating peripheral nerve

Project description:We provide ChIP-Seq analysis of Egr2 and Sox10 transcription factor binding in Schwann cells of rat peripheral nerve ChIP-Seq analysis of Egr2 and Sox10 binding in P15 rat sciatic nerve. Wiggle files of negative log of posterior probability determined by Mosaics.

2012-04-01 | E-GEOD-35132 | biostudies-arrayexpress

Genome-wide analysis of EGR2/SOX10 binding in myelinating peripheral nerve

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Project description:Egr2/Krox20 and Sox10 regulate genes involved in formation of myelination in the peripheral nervous system. ChIP-chip assays were performed on rat sciatic nerve at P15, a peak timepoint of myelination. In addition, Faire was used to identify areas of open chromatin. This experiment includes a custom ChIP-chip design incorporating many genes that are dynamically regulated during peripheral nerve myelination. Two antibodies were used for Egr2, Abcam and Covance PRB-236P. Egr2 and Sox10 ChIP samples were hybridized along with total input. In addition, FAIRE samples were hybridized relative to input DNA

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Project description:Sciatic nerve FAIRE-chip and ChIP-Chip with Egr2/Krox20 and Sox10

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Project description:ChIP-seq of Sox10 in spinal cord and sciatic nerve was performed to determine shared and unique binding sites for Sox10 in oligodendrocytes and Schwann cells, respectively. Sox10 is required for both Schwann cell and oligodendrocyte development. In addition, differentiation of myelinating glia is dependent upon axonal signaling, so these studies were performed in vivo.

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Project description:Enhancer Analysis in Peripheral Nerve: ChIP-seq of H3K27ac in P15 sciatic nerve

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