Project description:Knock-down of LSD1 using siRNA approach induced regulation of several proliferation-associated genes in ER-negative breast cancer cells MDA-MB-231.
Project description:We describe here the proteome of the human breast cancer cell line MDA-MB-231 expressing normal levels of the lncRNA NORAD, or after knock-down NORAD with siRNA and LNA gapmers.
Project description:Knock-down of LSD1 using siRNA approach induced regulation of several proliferation-associated genes in ER-negative breast cancer cells MDA-MB-231. To identify changes on gene expression caused by treatment with siRNA directed against LSD1 (si) or control siRNA (control) in MDA-MB-231 cells, total RNA was purified from the cells after treatment for 6 days (2 rounds of transfection). Three biological replicates were used.
Project description:Transcriptional profiling of MDA-MB-231 comparing the siRNA-mediated HNRPA2B1 knock-downs versus mock-transfected controls Three siRNA vs mock transfections
Project description:Transcriptional profiling of MDA-MB-231 samples taken at 0, 1, 2 and 4hr timepoints upon exposure to alpha-amanitine, comparing the siRNA-mediated HNRPA2B1 knock-downs versus mock-transfected controls
Project description:Transcriptional profiling of MDA-MB-231 samples taken at 0, 1, 2 and 4hr timepoints upon exposure to alpha-amanitine, comparing the siRNA-mediated HNRPA2B1 knock-downs versus mock-transfected controls 4 biological replicates for each time point for the siRNA transfections and controls respectively
Project description:RhoGDIbeta (ARHGDIB) is often expressed in tumor cells. It negatively regulates Rho-GTPases, but may have other functions as well. To analyze its effect on gene expression, RhoGDIbeta was suppressed by RNA interference in MDA-MB-231 breast cancer cells and changes in gene expression monitored by cDNA microarrays. Keywords: siRNA knock-down analysis
