Project description:Proteome characterization of isolated mitochondria samples prepared from three commonly used acute leukemia cell lines (HL-60, KG-1, MV-4-11). Data were compared to isolated mitochondria prepared from peripheral blood mononuclear cells (PBMC), isolated from healthy human subjects.
Project description:To access different gene expression between hepatic diseases patients and healthy adults, we collected peripheral blood samples to abstract PBMC. A total of 15 patients and 5 healthy adults were recruited.
Project description:Whole blood was collected from healthy and autistic infants and peripheral blood mononuclear cells (PBMC) were isolated. Transcriptional profile in PBMCs was compared between healthy and autistic infants.
Project description:Aberrant gene expression analysis between peripheral blood mononuclear cell (PBMC) samples from healthy individuals and patients with pancreatic carcinoma, gastric carcinoma and hepatocellular carcinoma (HCC) were identified using Affymetrix gene arrays. Peripheral blood mononuclear cell (PBMC) from healthy individuals, patients with pancreatic carcinoma, gastric carcinoma and HCC were isolated and total RNA was extracted for Affymetrix gene microarray analysis.
Project description:In a randomized controlled dietary intervention study we compared an isocaloric Healthy Nordic diet with the average Nordic diet for influence on peripheral blood mononuclear cells (PBMC) gene expression. We studied obese adults with features of the metabolic syndrom, n=66. There was no significant difference in age, BMI, or gene expression between the groups before the intervention. The intervention lasted for 18-24 weeks.
Project description:Aberrant gene expression analysis between peripheral blood mononuclear cell (PBMC) samples from healthy individuals and patients with chronic hepatitis B carriers and HCC were identified using Affymetrix gene arrays.M-BM- Peripheral blood mononuclear cell (PBMC) from healthy individuals, patients with patients with chronic hepatitis B carriers and HCC were isolated and total RNA was extracted for Affymetrix gene microarray analysis.
Project description:Whole blood was collected from healthy adult and peripheral blood mononuclear cells (PBMC) were isolated. PBMCs were cultured in medium or stimulated with RNase treated EC-12 or untreated EC-12 for 6hours. Then transcriptome profiles in PBMC were obtained.
Project description:We performed systems analyses of immune responses to the meningococcal polysaccharide (MPSV4) and conjugate (MCV4) vaccines in healthy adults. The goal was to identify innate gene signatures that correlate to antibody responses induced by these vaccines. Healthy adult volunteers (18-45 years of age) were randomized to be vaccinated subcutaneously with MPSV4 (Menomune®, n=13) or intramuscularly with MCV4 (MenactraTM, n=17) (Sanofi Pasteur, Swiftwater, PA). Whole blood samples were collected using CPT tubes at days 0, 3 and 7 post-vaccination. Peripheral blood mononuclear cells (PBMC) were isolated from fresh blood and used for DNA microarray experiments.
Project description:Aberrant gene expression analysis between peripheral blood mononuclear cell (PBMC) samples from healthy individuals and patients with chronic hepatitis B carriers and HCC were identified using Affymetrix gene arrays.