Project description:Real-time quantitative PCR analysis of microRNA expression in neonatal cardiovascular progenitors before and after 7 Days of simulated microgravity.
Project description:Human cardiovascular progenitors were isolated from neonatal (<1 month old) atrial tissue and cloned by single cell dilution. MicroRNA expression in neonatal cardiovascular progenitor cell (CPC) clones was compared before and after exposure to simulated microgravity using sabiosciences cell development & differentiation miRNA PCR array.
Project description:Human cardiovascular stem cells were isolated from adult (57-75 year old) and neonatal (<1 month old) atrial tissue. Cardiovascular stem cells were then cloned by single cell dilution and compared using sabiosciences cell development & differentiation miRNA PCR array. microRNA expression profiling by RT-PCR, 3 cardiac progenitor cell clones isolated from adults were run separately and results were pooled and compared to 8 neonatal cardiovascular progenitor cell clones that were run separately and pooled.
