Project description:To analyze cell lineage specific global transcriptional changes during retinogenesis, we sequenced mRNA from developing retina fractioned to Cd73+ photoreceptors and Cd73- other cells, in addition to the whole retina at embryonic period.
Project description:During retinal development, progenitor cells give rise to six different types of neurons, and one glial cell. This process requires the expression of genes that confer specific functions and identity to each cell. Previous works have reported the miRNAs expression profile in retina, but is still necessary to further define individual retinal cell populations profiles. We isolated postmitotic mice CD73-positive Rods, Müller glial cells and Retinal Progenitors Cells (E17.5); we then analyzed their miRNA profile expression by microarrays. Using wild type mice we FACS-isolated postmitotic CD73-positive Rods (posnatal day 5). We isolated primary Müller glia cultures from postnatal day 8 mice and retinal progenitors cells from E17.5 mouse embryos; we then sent the samples to the gene expression unit at Instituto de Medicina Genomica (INMEGEN), to analyze their miRNA profile expression by microarrays.
Project description:MSCs are a heterogeneous population and the specific population of MSCs may exhibit a selective ability for tissue repair. The aim of our research was to adapt the CD73+ subgroup of adipose derived MSCs (AD-MSCs) for the therapy of myocardial infarction (MI). Our results revealed that CD73+ AD-MSCs played more effective role in the acceleration function of cardiac recovery by promoting angiogenesis in a rat model of MI compared to mixed AD-MSCs and CD73- AD-MSCs. Microarray analysis shows differences between CD73+ and CD73- AD-MSCs when transcription profile of these two subgroups were compared, especially in VEGF pathway.
Project description:During retinal development, progenitor cells give rise to six different types of neurons, and one glial cell. This process requires the expression of genes that confer specific functions and identity to each cell. Previous works have reported the miRNAs expression profile in retina, but is still necessary to further define individual retinal cell populations profiles. We isolated postmitotic mice CD73-positive Rods, Müller glial cells and Retinal Progenitors Cells (E17.5); we then analyzed their miRNA profile expression by microarrays.
Project description:To examine the gene expression data in the retina in HIV positive and HIV negative autopsy eyes we used a microarray approach with the HumanHT-12 v4 Expression BeadChip.<br><br><br><br>Retinal punches were made for the following regions<br><br><br><br>CWS = cotton wool spot<br><br>IRH = intra-retinal hemorrhage<br><br>PP = posterior pole<br><br>