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Genome editing reveals a role for OCT4 in human preimplantation development [MiSeq]

ABSTRACT: Genome editing reveals a role for OCT4 in human preimplantation development [MiSeq]

PROVIDER: PRJNA392721 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5985240_1.fastq.gz	Fastqsanger.gz
	SRR5985240_2.fastq.gz	Fastqsanger.gz
	SRR5985250_1.fastq.gz	Fastqsanger.gz
	SRR5985250_2.fastq.gz	Fastqsanger.gz
	SRR5985260_1.fastq.gz	Fastqsanger.gz

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Genome editing reveals a role for OCT4 in human preimplantation development [MiSeq]

Project description:Despite their fundamental biological and clinical importance, the molecular mechanisms that regulate the first cell fate decisions in the human embryo are not well understood. Here we use CRISPR?Cas9-mediated genome editing to investigate the function of the pluripotency transcription factor OCT4 during human embryogenesis. We identified an efficient OCT4-targeting guide RNA using an inducible human embryonic stem cell-based system and microinjection of mouse zygotes. Using these refined methods, we efficiently and specifically targeted the gene encoding OCT4 (POU5F1) in diploid human zygotes and found that blastocyst development was compromised. Transcriptomics analysis revealed that, in POU5F1-null cells, gene expression was downregulated not only for extra-embryonic trophectoderm genes, such as CDX2, but also for regulators of the pluripotent epiblast, including NANOG. By contrast, Pou5f1-null mouse embryos maintained the expression of orthologous genes, and blastocys t development was established, but maintenance was compromised. We conclude that CRISPR?Cas9-mediated genome editing is a powerful method for investigating gene function in the context of human development.

2017-09-21 | GSE100119 | GEO

Identification of Oct4-dependent genes in vivo

Project description:Transcriptome analysis of Oct4 null and wild type preimplantation mouse embryos by RNA-sequencing

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The Role and Mechanism of TEAD4 in Preimplantation Embryonic Development in Mice and Cattle

Project description:Tead4 is a critical transcription factor expressed during preimplantation development and is essential for the expression of trophectoderm-specific genes in mice. However, the mechanism of Tead4’s function in mouse preimplantation development and its conservation across mammals remains unclear. Here, we report that Tead4 is a crucial transcription factor necessary for blastocyst formation in mice. Disruption of TEAD4 through base editing results in developmental arrest at the morula stage. Additionally, RNA-seq analysis reveals dysregulation of 670 genes in Tead4 knockout embryos. As anticipated, Tead4 knockout leads to a decrease in trophectoderm genes CDX2 and GATA3. Intriguingly, we observed a reduction in KRT8, suggesting that Tead4 influences the integrity of the trophectoderm epithelium in mice. More importantly, we noted a decrease in nuclear YAP for Tead4-deficient embryos, indicating that Tead4 directly regulates Hippo signaling. In contrast, bovine embryos with TEAD4 depletion can still develop to blastocysts with normal expression of CDX2, GATA3, and SOX2, albeit with a decrease in total cell number and ICM cell number. In conclusion, we propose that Tead4 regulates mouse blastocyst formation via Krt8 and Yap, both of which are critical regulators of mouse preimplantation development.

2024-07-15 | GSE237418 | GEO

Identification of Oct4-dependent genes in vivo

Project description:Transcriptome analysis of Oct4 null and wild type preimplantation mouse embryos by RNA-sequencing N=3 embryos of two genotypes (+/+ or -/-) at each of three time points (E2.5, E3.75, E4.5) were sequenced.

2013-06-06 | E-GEOD-47089 | biostudies-arrayexpress

Expression data from preimplantation mouse embryos

Project description:Landmark events occur in a coordinated manner during preimplantation development of the mammalian embryo, yet the regulatory network that orchestrates these events remains largely unknown. We delineated the regulons of Oct4, Sall4, and Nanog using morpholino (MO)-mediated gene knockdowns followed by microarray analysis of pooled embryos.

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MiSeq paired-end sequencing of HEK293T cells stably expressing a Cas9/gRNA-dependent editable reporter vector to test novel nucleobase-editing techniques

Project description:The goal of these experiments were to test the on-target and target-adjacent editing efficiencies of different single-nucleobase editing systems. Previous studies have shown that tethering DNA mutating enzymes to Cas9-nickase-UGI complexes results in editing of chromosomal DNA. However, these editing events encompass undesirable target-adjacent nucleobase edits. Here, we characterize a novel approach that reduces the frequency of target-adjacent editing while maintaining a high level of on-target editing.

2020-02-23 | E-MTAB-8742 | biostudies-arrayexpress

Transcriptome of mouse preimplantation development

Project description:Transcriptome of mouse preimplantation development

| PRJNA291206 | ENA

Expression data from preimplantation mouse embryos

2012-11-09 | E-GEOD-42135 | biostudies-arrayexpress

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Project description:Evaluation of the transcriptomic profile of the rabbit embryo along the preimplantation period during in vivo development. Three embryonic stages were used: four cell embryos (H32 post-coïtum); morula (H58 pc) and blastocyst (H90 pc). Keywords: time course rabbit embryo 18 samples

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2023-02-27 | GSE225711 | GEO

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