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Expression data from PolyIC treated EndoC-ßH1 cells

ABSTRACT: Expression data from PolyIC treated EndoC-ßH1 cells

PROVIDER: PRJNA412004 | ENA |

REPOSITORIES: ENA

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Expression data from PolyIC treated EndoC-ßH1 cells

Project description:Type 1 diabetes (T1D) is a chronic disease characterized by an autoimmune-mediated destruction of insulin-producing pancreatic β cells. Environmental factors such as viruses play an important role in the onset of T1D and interact with predisposing genes. Recent data suggest that viral infection of human islets leads to a decrease in insulin production rather than β cell death, suggesting loss of β cell identity. We undertook this study to examine whether viral infection could induce human ß cell dedifferentiation. Using the functional human β cell line EndoC-βH1, we demonstrate that polyinosinic-polycitidilic acid (PolyI:C), a synthetic double-stranded RNA that mimics a by-product of viral replication induces a decrease in β cell-specific gene expression. In parallel to this loss, the expression of progenitor-like genes such as SOX9 was activated following PolyI:C treatment or enteroviral infection. SOX9 was induced by the NF-kB pathway and also in a paracrine non-cell autonomous fashion through the secretion of IFNA. Finally, we identified new SOX9 targets in human β cells as new markers of dedifferentiation in T1D. These findings reveal that inflammatory signaling has clear implications in human β cell dedifferentiation.

2018-01-31 | GSE104190 | GEO

EndoC-betaH1 cells treated with interferon alpha

Project description:EndoC-betaH1 cells were treated with 2000 U/mL interferon alpha for 8 or 24h and submitted to proteomic analysis.

2020-04-15 | PXD014244 | Pride

Expression data from SOX9 overexpressing EndoC-ßH1 cells

Project description:Expression data from SOX9 overexpressing EndoC-ßH1 cells

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Transciptional profiling of polyIC-treated or untreated, polyIC-experience or naive primary murine alveolar macrophages

Project description:Transciptional profiling of polyIC-treated or untreated, polyIC-experience or naive primary murine alveolar macrophages

| PRJNA1103797 | ENA

Proteomics analysis of EndoC-betaH1 cells treated with IL-1b and IFN-g

Project description:EndoC-betaH1 cells were treated with IL-1b and IFN-g for 48h, digested with trypsin, multiplexed with TMT-10, fractionated by high pH reverse-phase chromatography and analyzed by tandem LC-MS/MS.

2019-09-26 | PXD011902 | Pride

Expression data from SOX9 overexpressing EndoC-ßH1 cells

Project description:Human ß cell dedifferentiation as a potent mechanism of diabetes is gaining prominence. Several data suggest an upregulation of the transcription factor SOX9, a progenitor and duct cell marker during ß cell dedifferentiation. However, its targets in such cells need more understanding. Here, we overexpressed SOX9 and a constitutively active mutant (VP16-SOX9∆TAD) in Human pancreatic beta EndoC-ßH1 cells in order to understand its targets.

2018-01-31 | GSE104195 | GEO

Transcriptome profile of human pancreatic beta cell line (EndoC-M-NM-2H2 ) following knockdown of RFX6.

Project description:RFX6 is a key transcription factor for the development of mouse pancreas, however the functional roles of RFX6 in human beta cells are poorly explored. Thus transcriptome analysis was perfomed to determine the functional targets of RFX6 in human beta cells using the recently developed human beta cell-line EndoC-M-NM-2H2. Transcriptome profile of human beta cell line (EndoC-M-NM-2H2 cells) following siRNA induced knockdown of RFX6 is compared with siControl (siNT) treated EndoC-M-NM-2H2 cells.

2014-12-22 | E-GEOD-59049 | biostudies-arrayexpress

PCSK9 knockdown in EndoC-bH1 cells at 72 hours

Project description:The aim of the study was to characterize the role of PCSK9 in human beta cells. We performed siRNA-mediated knockdown of PCSK9 in human beta cell line EndoC-bH1 and compared the expression profiles against control siRNA-treated cells.

2022-03-31 | GSE182016 | GEO

AmpliSeq Transcriptome Analysis of cells stimulated with polyIC in presence of GFP or NS5 protein

Project description:We compared polyIC stimulated cells in the presence of either GFP or NS5 protein

2018-08-18 | GSE118686 | GEO

Characterization of EndoC-BH5 a novel human pancreatic beta cell line. Comparative expression profile after stimulation stimulation with Interferon gamma and interleukin 1b.

Project description:Responsiveness of EndoC-BH5 cells to cytokines was examined by RNA-seq of cells treated with IFNγ and IL1β for 24 h. The treatment resulted in profound changes in their transcriptome and upregulation of genes involved in the inflammatory pathwayand antigen processing and presentation, similar to previous studies on EndoC-BH1 cells and adult human islets. EndoC-BH5 cells hence represent a powerful tool to investigate the dialogue between beta cells and the immune system.

2023-07-20 | GSE224731 | GEO

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