Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

PCSK9 knockdown in EndoC-bH1 cells at 72 hours

ABSTRACT: The aim of the study was to characterize the role of PCSK9 in human beta cells. We performed siRNA-mediated knockdown of PCSK9 in human beta cell line EndoC-bH1 and compared the expression profiles against control siRNA-treated cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE182016 | GEO | 2022/03/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

PSCK9 knockdown in EndoC-bH1 cells at 72 h (DIA proteomics experiment)

Project description:EndoC-bH1 cells were incubated with control siRNA or PCSK9 siRNA for 72 hours. The experiment was powered based PCSK9 knockdown in a pilot experiment. Sample size: 12x12.

2022-04-07 | PXD027911 | Pride

PSCK9 knockdown in EndoC-bH1 cells at 72 h (DIA secretome experiment)

Project description:EndoC-bH1 cells were incubated with control siRNA or PCSK9 siRNA for 72 hours. The experiment was powered based PCSK9 knockdown in the pilot experiment [PXDxxx (PE083)]. Sample size: 12x12.

2022-04-07 | PXD027913 | Pride

PSCK9 knockdown in EndoC-bH1 cells at 72 h (pilot TMT experiment)

Project description:EndoC-bH1 cells were incubated with control siRNA or PCSK9 siRNA for 72 hours. Unpowered exploratory experiment. Sample size: 3x3.

2022-04-07 | PXD027921 | Pride

PCSK9 knockdown in EndoC-bH1 cells at 72 hours

Project description:PCSK9 knockdown in EndoC-bH1 cells at 72 hours

| PRJNA754177 | ENA

PSCK9 knockdown in EndoC-bH1 cells time course (DIA secretome time course)

Project description:EndoC-bH1 cells were incubated with control siRNA or PCSK9 siRNA for 24, 48 and 72 hours. Untreated baseline samples were also included. Unpowered exploratory experiment. Sample size: triplicate per condition.

2022-04-07 | PXD027920 | Pride

endoC-bH1

Project description:Gene expression in EndoC-bH1 human beta-cell line

| PRJEB15283 | ENA

Effect of control siRNA knockdown on EndoC-bH1 cell lysate proteome (pilot TMT experiment)

Project description:EndoC-bH1 cells proteomics was assessed in untreated baseline condition and after treatment with control siRNA at 24, 48 and 72 hours. Unpowered exploratory experiment. Sample size: triplicate per condition.

2022-04-07 | PXD027898 | Pride

RNA-Sequencing of EndoC-BH1 cells Overexpressing OAS1, OAS2, or OAS3

Project description:EndoC-BH1 cells were plated in normal media and forward transfected with lipofectamine 3000 (2ug cDNA) of empty, OAS1, OAS2, or OAS3 plasmids from Vector builder

2024-01-01 | GSE244273 | GEO

Transcriptome profile of human pancreatic beta cell line (EndoC-M-NM-2H2 ) following knockdown of RFX6.

Project description:RFX6 is a key transcription factor for the development of mouse pancreas, however the functional roles of RFX6 in human beta cells are poorly explored. Thus transcriptome analysis was perfomed to determine the functional targets of RFX6 in human beta cells using the recently developed human beta cell-line EndoC-M-NM-2H2. Transcriptome profile of human beta cell line (EndoC-M-NM-2H2 cells) following siRNA induced knockdown of RFX6 is compared with siControl (siNT) treated EndoC-M-NM-2H2 cells.

2014-12-22 | E-GEOD-59049 | biostudies-arrayexpress

Characterization of EndoC-BH5 a novel human pancreatic beta cell line. Comparative expression profile after stimulation stimulation with Interferon gamma and interleukin 1b.

Project description:Responsiveness of EndoC-BH5 cells to cytokines was examined by RNA-seq of cells treated with IFNγ and IL1β for 24 h. The treatment resulted in profound changes in their transcriptome and upregulation of genes involved in the inflammatory pathwayand antigen processing and presentation, similar to previous studies on EndoC-BH1 cells and adult human islets. EndoC-BH5 cells hence represent a powerful tool to investigate the dialogue between beta cells and the immune system.

2023-07-20 | GSE224731 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data