Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The histone demethylase Kdm6 regulates a mature gene expression program in differentiating cerebellar granule neurons

ABSTRACT: The histone demethylase Kdm6 regulates a mature gene expression program in differentiating cerebellar granule neurons

PROVIDER: PRJNA415630 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6208963.fastq.gz	Fastqsanger.gz
	SRR6208964.fastq.gz	Fastqsanger.gz
	SRR6208965.fastq.gz	Fastqsanger.gz
	SRR6208966.fastq.gz	Fastqsanger.gz
	SRR6208967.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

The histone demethylase Kdm6 regulates a mature gene expression program in differentiating cerebellar granule neurons

Project description:The histone H3 lysine 27 (H3K27) demethylase Kdm6b (Jmjd3) can promote cellular differentiation, however its physiological functions in neurons remain to be fully determined. We studied the expression and function of Kdm6b in differentiating granule neurons of the developing postnatal mouse cerebellum. At postnatal day 7, Kdm6b is expressed throughout the layers of the developing cerebellar cortex, but its expression is upregulated in newborn cerebellar granule neurons (CGNs). Atoh1-Cre mediated conditional knockout of Kdm6b in CGN precursors either alone or in combination with Kdm6a did not disturb the gross morphological development of the cerebellum. Furthermore, RNAi-mediated knockdown of Kdm6b in cultured CGN precursors did not alter the induced expression of early neuronal marker genes upon cell cycle exit. By contrast, knockdown of Kdm6b significantly impaired the induction of a mature neuronal gene expression program, which includes gene products required for functional synapse maturation. Loss of Kdm6b also impaired the ability of Brain-Derived Neurotrophic Factor (BDNF) to induce expression of Grin2c and Tiam1 in maturing CGNs. Taken together, these data reveal a previously unknown role for Kdm6b in the postmitotic stages of CGN maturation and suggest that Kdm6b may work, at least in part, by a transcriptional mechanism that promotes gene sensitivity to regulation by BDNF.

2017-11-20 | GSE106120 | GEO

Gene expression of cerebellar granule cell layer

Project description:We performed gene-expression analysis of mouse cerebellar granule cell layer as compared to that of Purkinje cells. DNA microarray analysis detected genes in cerebellar granule cell layer, most of which are classified into functional molecule categories. Our comparative analysis between Purkinje cells and the granule cell layer showed that the characteristic expression pattern in Purkinje cells was particularly represented by M-bM-^@M-^\the neural communication systemM-bM-^@M-^] components. Pukinje cells and granule cell layer of the mouse cerebellum were collected by laser microdissection for RNA extraction and hybridization on Affymetrix microarrays.

2013-01-17 | E-GEOD-26750 | biostudies-arrayexpress

Gene expression of cerebellar granule cell layer

2013-01-17 | GSE26750 | GEO

The histone demethylase Kdm6b regulates a mature gene expression program in differentiating cerebellar granule neurons.

| S-EPMC5828961 | biostudies-literature

Notch signaling between cerebellar granule cell progenitors

Project description:During cerebellar development, maternal granule cell progenitors (GCPs) divide to produce not only postmitotic granule cells (GCs) but also sister GCPs. However, molecular machinery to proportionally produce distinct sister cell types from seemingly uniform GCPs is still elusive. Here we report Notch signaling makes a difference among GCPs, leading to their proportional differentiation.

2020-06-27 | GSE153313 | GEO

Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis [scRNA-seq]

Project description:To establish a new model system with which to study cerebellar granule lienage development and disease, human hbNES cells were differentiated to cerebellar granule neurons over a 56 day period.

2023-05-29 | GSE233338 | GEO

Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis [RNA-seq]

2023-05-29 | GSE233337 | GEO

Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis [ATAC-seq]

2023-05-29 | GSE233336 | GEO

WDR4 conditional knockout in mouse cerebellar granule progenitors for proteome analysis

Project description:To uncover candidates which are WDR4 downstream effectors regulating cerebellar granule neuron proliferation phenotype, we utilized quantitative proteomics analysis (TMT labeling) with granule neuron progenitors from P7 wdr4 conditional knockout (using Atoh1-Cre) and control cerebella.

2023-03-11 | PXD022319 | Pride

Notch signaling between cerebellar granule cell progenitors

Project description:Notch signaling between cerebellar granule cell progenitors

| PRJNA642138 | ENA