Project description:To investigate the mechanisms of the defense system during chicken embryo development, protein profiling of liver tissues in chicken embryo at was conducted. TMT was used to analyze the liver tissues proteomes with significantly different activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in chicken embryo.
Project description:We have profiled the gene expression in eight chicken embryo livers in the 11th day of incubation. Four eggs (labelled as WAT1-4) were injected with 0.15 ml/egg deionized water in days 9, 10 an 11 post incubation while four eggs (labeled CTR1-4) were kept as environmental controls. We found that water injections had a very small effect, regulating less than 1% of the genes. Both WAT and CTR data sets were used as references in further experiments for eggs expossed to various water-soluble carcinogenic compounds.
Project description:In order to investigate the chicken SLCO1B3 gene functin on the liver metabolism, we used the Yimeng blue eggshell and brown eggshell chickens as the chicken liver SLCO1B3 gene knock-down animal to do the proteomic analysis.
Project description:Transcriptional profiling of chicken embryo fibroblast (CEF) cells comparing CEF derived immortal chicken embryo fibroblast cell line (DF-1). Goal was to determine differentially expressed genes of CEF which were changed responding DF-1.
Project description:In the present study, we examined the hepatic transcriptome of chickens during the peri-hatch period—or the metabolic jump from chorioallantoic (embryo) to pulmonary (hatchling) respiration. Although our major interest was comparison of differentially-expressed genes between embryos and hatchlings, we made pairwise contrasts across six developmental ages. We collected the liver from four embryos at three ages (e16, e18 and e20) and four hatchling chicks at three ages (1, 3 and 9 days) post hatching. Liver samples (N=24) were used for extraction of total RNA which was then used for hybridization to 24 Affymetrix Chicken Genome Arrays. Ingenuity Pathways Analysis was used for functional annotation and mapping of differentially expressed (FDR≤0.05) genes to canonical pathways and gene interaction networks. We identified 1274 hepatic genes that were differentially expressed between embryos and hatchling chicks and of these, 284 genes are involved in lipid metabolism. The three most abundant found in liver of embryos were (MOGAT1, DIO3 and PDK4), whereas THRSP, FASN and DIO2 were greatly expressed in liver of hatchlings. Two functionally-distinct clusters of hepatic genes have emerged from our time-course transcriptional scans in the peri-hatch chicken. Cluster A genes are largely lipolytic with higher expression in the embryo, while Cluster B genes are mainly lipogenic and thermogenic with greater expression in liver of hatchlings. The present study describes the innate chorography of transcriptional responses of liver to the abrupt metabolic switch from aquatic ectothermy (embryos) to free-living endothermy (hatchling chicks).
Project description:Turkey embryos are very sensitive to perturbations in energy metabolism because they have a wider hatching window than chicken embryos. Mortality of turkey embryos during late-term incubation is high relative to chickens, and many surviving hatchlings have compromised vitality. Intestinal maturation at hatch is also crucial to survival and post-hatch performance. The study of poultry embryo metabolism during the last stages of incubation is difficult due to many shifts and changes that occur in preparation for hatching. Microarray technology is suitable to study complex biological systems like avian late-term embryonic development. Therefore, the objectives of this study were to create a customized focused oligonucleotide microarray based on chicken genome sequences that could be used to study late-term avian metabolism and intestinal maturation, and use this array to survey turkey embryos gene expression from 20 days of incubation until hatch. The key features of this microarray are that all genes present have been annotated and gene spot replication (4) within each array chip. Microarray analysis was performed on liver, pectoral muscle, hatching muscle, and duodenum Keywords: time course, embryo development
Project description:Red Jungle Fowl (male and female) tissues were analyzed using LC-MS/MS. Tissues analyzed were: adipose, adrenal gland, breast muscle, cerebellum, cerebrum, gonad, heart, hypothalamus, kidney, liver, lung, pancreas, proventriculus, sciatic nerve, speen. Samples were analyzed using an LTQ Velos Pro mass spectrometer. Xtandem was used to perform spectrum searches. Databases included are NCB refseq, Ensembl, and 6-frame translation of the chicken genome.
Project description:Transcriptional profiling of chicken embryo lung cells infected infectious laryngotracheitis virus (ILTV) comparing control uninfected lung cells. Goal was to determine the changes of host gene expression by ILTV infection and host-virus interaction.
Project description:Transcriptional profiling of chicken embryo lung cells infected infectious laryngotracheitis virus (ILTV) comparing control uninfected lung cells. Goal was to determine the changes of host gene expression by ILTV infection and host-virus interaction. Two-condition experiment, uninfected chicken embryo lung cells vs. infected chicken embryo lung cells. Biological replicates: 1 control uninfected sample, 1 infected experimental sample at each 1dpi, 3dpi, 5dpi, and 7 dpi.