Project description:B-cell development in the bone marrow proceeds through phenotypically defined stages. At the pre-B cell stage, cells stop cycling and rearrange the light chain of immunoglobulin. There are notable defects in the number of these cells and subsequent stages of B-cell development in mice with hematopoietic deficiency of the gene Ddx3x, when compared to wildtype control mice. We used microarrays to determine differences in gene expression between bone marrow pre-B cells in wild-type mice and those deficient in Ddx3x in hematopoietic cells (Vav1-cre x Ddx3x-floxed, male, hemizygous)
Project description:Triplicate RNA-seq expression analysis of bone marrow pre-B cells isolated from mice, to demonstrate repertoire at the IgH locus Triplicate RNA-seq expression analysis of bone marrow pre-B cells
Project description:RATIONALE: Radiation therapy uses high-energy x-rays to damage cancer cells. Drugs used in chemotherapy use different ways to stop cancer cells from dividing so they stop growing or die. Combining chemotherapy with bone marrow transplantation may allow the doctor to give higher doses of chemotherapy drugs and kill more tumor cells.
PURPOSE: Phase II trial to study the effectiveness of bone marrow transplantation in treating patients who have hematologic cancer.
Project description:Hematopoietic stem cells give rise to all blood lineages, can fully re-populate the bone marrow, and easily outlive the host organism. To better understand how stem cells remain fit during aging, we analyzed the proteome of hematopoietic stem and progenitor cells.
Project description:The bone marrow microenvironment of leukemia plays a key role in leukemia progression, but its molecular complexity in pre-B acute lymphoblastic leukemia (B-ALL), the most common cancer in children, remains poorly understood. Here we use single-cell RNA sequencing to characterise the bone marrow microenvironment during B-ALL progression.
Project description:Type 2 pre-cDCs are considered as a homogeneous population in the bone marrow. We used single cell RNA sequencing to show that the bone marrow contains two subsets of pre-cDC2s that are commited to give rise to different different cDC2 subsets in peripheral tissues.
Project description:Gene expression of mice bone marrow pre-B cells from both control and Vav-Cre Mef2cfl/fl mice (9 months old) We used these microarrays to identify the function of MEF2C in BM B lymphoid progenitors